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dc.contributor.authorjoshi, lovleen
dc.contributor.authorMali, BL
dc.contributor.authorGeddes, CD
dc.contributor.authorBaillie, L
dc.date.accessioned2017-08-16T16:26:58Z
dc.date.available2017-08-16T16:26:58Z
dc.date.issued2014-08-27
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.otherARTN e104334
dc.identifier.urihttp://hdl.handle.net/10026.1/9835
dc.description.abstract

Clostridium difficile is the primary cause of antibiotic associated diarrhea in humans and is a significant cause of morbidity and mortality. Thus the rapid and accurate identification of this pathogen in clinical samples, such as feces, is a key step in reducing the devastating impact of this disease. The bacterium produces two toxins, A and B, which are thought to be responsible for the majority of the pathology associated with the disease, although the relative contribution of each is currently a subject of debate. For this reason we have developed a rapid detection assay based on microwave-accelerated metal-enhanced fluorescence which is capable of detecting the presence of 10 bacteria in unprocessed human feces within 40 seconds. These promising results suggest that this prototype biosensor has the potential to be developed into a rapid, point of care, real time diagnostic assay for C. difficile.

dc.format.extente104334-e104334
dc.format.mediumElectronic-eCollection
dc.languageen
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.subjectBacterial Toxins
dc.subjectBiological Assay
dc.subjectClostridioides difficile
dc.subjectDNA Probes
dc.subjectDigoxigenin
dc.subjectEnterocolitis, Pseudomembranous
dc.subjectEnterotoxins
dc.subjectFeces
dc.subjectFluorescent Dyes
dc.subjectHumans
dc.subjectHydrazines
dc.subjectLimit of Detection
dc.subjectMicrowaves
dc.subjectOrganic Chemicals
dc.subjectPolymerase Chain Reaction
dc.subjectSpores, Bacterial
dc.titleExtraction and Sensitive Detection of Toxins A and B from the Human Pathogen Clostridium difficile in 40 Seconds Using Microwave-Accelerated Metal-Enhanced Fluorescence
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, N.I.H., Extramural
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000340880900011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue8
plymouth.volume9
plymouth.publication-statusPublished online
plymouth.journalPLoS ONE
dc.identifier.doi10.1371/journal.pone.0104334
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dcterms.dateAccepted2014-07-10
dc.identifier.eissn1932-6203
dc.rights.embargoperiodNo embargo
rioxxterms.versionofrecord10.1371/journal.pone.0104334
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2014
rioxxterms.typeJournal Article/Review


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