An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment
dc.contributor.author | 1. Teague, J | |
dc.contributor.author | Scott, T | |
dc.contributor.author | sharma, sanjay | |
dc.contributor.author | George, G | |
dc.contributor.author | Allen, M | |
dc.date.accessioned | 2017-06-07T08:43:13Z | |
dc.date.available | 2017-06-07T08:43:13Z | |
dc.date.issued | 2017-06-02 | |
dc.identifier.issn | 2077-1312 | |
dc.identifier.issn | 2077-1312 | |
dc.identifier.other | ARTN 22 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/9433 | |
dc.description.abstract |
The development of low-cost, open-source Remotely Operated Vehicle (ROV) systems has provided almost unrestricted access for researchers looking to monitor the marine environment in ever greater resolution. Sampling microbial communities from the marine environment, however, still usually relies on Niskin-bottle sampling (ROV or Conductivity-Temperature-Depth sampler (CTD) based), a method which introduces an inaccuracy and variability that is incompatible with metatranscriptomic analysis, for example. Here, we describe a versatile, easily-replicated platform which achieves in situ mRNA preservation, via the addition of RNAlater to filtered microbial cells, to enhance ROV or CTD functionality. | |
dc.format.extent | 22-22 | |
dc.language.iso | en | |
dc.publisher | Journal of Marine Science and Engineering | |
dc.subject | remotely operated vehicle | |
dc.subject | metatranscriptomics | |
dc.subject | Niskin | |
dc.title | An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment | |
dc.type | report | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000423689700008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.confidential | false | |
plymouth.publication-status | Published online | |
dc.identifier.doi | 10.3390/jmse5020022 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Engineering, Computing and Mathematics | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA12 Engineering | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Marine Institute | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.identifier.eissn | 2077-1312 | |
dc.rights.embargoperiod | No embargo | |
rioxxterms.versionofrecord | 10.3390/jmse5020022 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Technical Report |