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dc.contributor.authorEyres, Ien
dc.contributor.authorBoschetti, Cen
dc.contributor.authorCrisp, Aen
dc.contributor.authorSmith, TPen
dc.contributor.authorFontaneto, Den
dc.contributor.authorTunnacliffe, Aen
dc.contributor.authorBarraclough, TGen
dc.date.accessioned2017-06-02T10:16:29Z
dc.date.available2017-06-02T10:16:29Z
dc.date.issued2015-11-04en
dc.identifier.urihttp://hdl.handle.net/10026.1/9406
dc.description.abstract

BACKGROUND: Although prevalent in prokaryotes, horizontal gene transfer (HGT) is rarer in multicellular eukaryotes. Bdelloid rotifers are microscopic animals that contain a higher proportion of horizontally transferred, non-metazoan genes in their genomes than typical of animals. It has been hypothesized that bdelloids incorporate foreign DNA when they repair their chromosomes following double-strand breaks caused by desiccation. HGT might thereby contribute to species divergence and adaptation, as in prokaryotes. If so, we expect that species should differ in their complement of foreign genes, rather than sharing the same set of foreign genes inherited from a common ancestor. Furthermore, there should be more foreign genes in species that desiccate more frequently. We tested these hypotheses by surveying HGT in four congeneric species of bdelloids from different habitats: two from permanent aquatic habitats and two from temporary aquatic habitats that desiccate regularly. RESULTS: Transcriptomes of all four species contain many genes with a closer match to non-metazoan genes than to metazoan genes. Whole genome sequencing of one species confirmed the presence of these foreign genes in the genome. Nearly half of foreign genes are shared between all four species and an outgroup from another family, but many hundreds are unique to particular species, which indicates that HGT is ongoing. Using a dated phylogeny, we estimate an average of 12.8 gains versus 2.0 losses of foreign genes per million years. Consistent with the desiccation hypothesis, the level of HGT is higher in the species that experience regular desiccation events than those that do not. However, HGT still contributed hundreds of foreign genes to the species from permanently aquatic habitats. Foreign genes were mainly enzymes with various annotated functions that include catabolism of complex polysaccharides and stress responses. We found evidence of differential loss of ancestral foreign genes previously associated with desiccation protection in the two non-desiccating species. CONCLUSIONS: Nearly half of foreign genes were acquired before the divergence of bdelloid families over 60 Mya. Nonetheless, HGT is ongoing in bdelloids and has contributed to putative functional differences among species. Variation among our study species is consistent with the hypothesis that desiccating habitats promote HGT.

en
dc.format.extent90 - ?en
dc.languageengen
dc.language.isoengen
dc.subjectAnimalsen
dc.subjectDesiccationen
dc.subjectEcosystemen
dc.subjectGene Transfer, Horizontalen
dc.subjectMolecular Sequence Dataen
dc.subjectPhylogenyen
dc.subjectRotiferaen
dc.subjectSequence Analysis, DNAen
dc.subjectSpecies Specificityen
dc.titleHorizontal gene transfer in bdelloid rotifers is ancient, ongoing and more frequent in species from desiccating habitats.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/26537913en
plymouth.volume13en
plymouth.publication-statusPublished onlineen
plymouth.journalBMC Biolen
dc.identifier.doi10.1186/s12915-015-0202-9en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA07 Earth Systems and Environmental Sciences
dc.publisher.placeEnglanden
dcterms.dateAccepted2015-10-20en
dc.identifier.eissn1741-7007en
dc.rights.embargoperiodNo embargoen
rioxxterms.versionofrecord10.1186/s12915-015-0202-9en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-11-04en
rioxxterms.typeJournal Article/Reviewen


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