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dc.contributor.authorLittlejohn, GRen
dc.contributor.authorGouveia, JDen
dc.contributor.authorEdner, Cen
dc.contributor.authorSmirnoff, Nen
dc.contributor.authorLove, Jen
dc.date.accessioned2017-05-24T19:26:08Z
dc.date.available2017-05-24T19:26:08Z
dc.date.issued2010-06en
dc.identifier.urihttp://hdl.handle.net/10026.1/9344
dc.description.abstract

*Air spaces in the leaf mesophyll generate deleterious optical effects that compromise confocal microscopy. *Leaves were mounted in the nontoxic, nonfluorescent perfluorocarbon, perfluorodecalin (PFD), and optical enhancement and physiological effect were assessed using confocal microscopy and chlorophyll fluorescence. *Mounting leaves of Arabidopsis thaliana in PFD significantly improved the optical qualities of the leaf, thereby enabling high-resolution laser scanning confocal imaging over twofold deeper into the mesophyll, compared with using water. Incubation in PFD had less physiological impact on the mounted specimen than water. *We conclude that the application of PFD as a mounting medium substantially increases confocal image resolution of living mesophyll and vascular bundle cells, with minimal physiological impact.

en
dc.format.extent1018 - 1025en
dc.languageengen
dc.language.isoengen
dc.subjectArabidopsisen
dc.subjectBiological Transporten
dc.subjectFluorocarbonsen
dc.subjectGerminationen
dc.subjectImaging, Three-Dimensionalen
dc.subjectMicroscopy, Confocalen
dc.subjectPhotosystem II Protein Complexen
dc.subjectPlant Leavesen
dc.subjectSeedlingsen
dc.subjectSpectrometry, Fluorescenceen
dc.titlePerfluorodecalin enhances in vivo confocal microscopy resolution of Arabidopsis thaliana mesophyll.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/20374500en
plymouth.issue4en
plymouth.volume186en
plymouth.publication-statusPublisheden
plymouth.journalNew Phytolen
dc.identifier.doi10.1111/j.1469-8137.2010.03244.xen
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
dc.publisher.placeEnglanden
dc.identifier.eissn1469-8137en
dc.rights.embargoperiodNo embargoen
rioxxterms.versionofrecord10.1111/j.1469-8137.2010.03244.xen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


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