Perfluorodecalin enhances <i>in vivo</i> confocal microscopy resolution of <i>Arabidopsis thaliana</i> mesophyll
dc.contributor.author | Littlejohn, George | |
dc.contributor.author | Gouveia, JD | |
dc.contributor.author | Edner, C | |
dc.contributor.author | Smirnoff, N | |
dc.contributor.author | Love, J | |
dc.date.accessioned | 2017-05-24T19:26:08Z | |
dc.date.available | 2017-05-24T19:26:08Z | |
dc.date.issued | 2010-06 | |
dc.identifier.issn | 0028-646X | |
dc.identifier.issn | 1469-8137 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/9344 | |
dc.description.abstract |
*Air spaces in the leaf mesophyll generate deleterious optical effects that compromise confocal microscopy. *Leaves were mounted in the nontoxic, nonfluorescent perfluorocarbon, perfluorodecalin (PFD), and optical enhancement and physiological effect were assessed using confocal microscopy and chlorophyll fluorescence. *Mounting leaves of Arabidopsis thaliana in PFD significantly improved the optical qualities of the leaf, thereby enabling high-resolution laser scanning confocal imaging over twofold deeper into the mesophyll, compared with using water. Incubation in PFD had less physiological impact on the mounted specimen than water. *We conclude that the application of PFD as a mounting medium substantially increases confocal image resolution of living mesophyll and vascular bundle cells, with minimal physiological impact. | |
dc.format.extent | 1018-1025 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Wiley | |
dc.subject | Arabidopsis | |
dc.subject | confocal microscopy | |
dc.subject | fluorescence | |
dc.subject | mesophyll | |
dc.subject | perfluorocarbon | |
dc.subject | perfluorodecalin | |
dc.title | Perfluorodecalin enhances <i>in vivo</i> confocal microscopy resolution of <i>Arabidopsis thaliana</i> mesophyll | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000277526900022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 4 | |
plymouth.volume | 186 | |
plymouth.publication-status | Published | |
plymouth.journal | New Phytologist | |
dc.identifier.doi | 10.1111/j.1469-8137.2010.03244.x | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | England | |
dc.identifier.eissn | 1469-8137 | |
dc.rights.embargoperiod | No embargo | |
rioxxterms.versionofrecord | 10.1111/j.1469-8137.2010.03244.x | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review |