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dc.contributor.authorLittlejohn, GR
dc.contributor.authorGouveia, JD
dc.contributor.authorEdner, C
dc.contributor.authorSmirnoff, N
dc.contributor.authorLove, J
dc.date.accessioned2017-05-24T19:26:08Z
dc.date.available2017-05-24T19:26:08Z
dc.date.issued2010-06
dc.identifier.issn0028-646X
dc.identifier.issn1469-8137
dc.identifier.urihttp://hdl.handle.net/10026.1/9344
dc.description.abstract

*Air spaces in the leaf mesophyll generate deleterious optical effects that compromise confocal microscopy. *Leaves were mounted in the nontoxic, nonfluorescent perfluorocarbon, perfluorodecalin (PFD), and optical enhancement and physiological effect were assessed using confocal microscopy and chlorophyll fluorescence. *Mounting leaves of Arabidopsis thaliana in PFD significantly improved the optical qualities of the leaf, thereby enabling high-resolution laser scanning confocal imaging over twofold deeper into the mesophyll, compared with using water. Incubation in PFD had less physiological impact on the mounted specimen than water. *We conclude that the application of PFD as a mounting medium substantially increases confocal image resolution of living mesophyll and vascular bundle cells, with minimal physiological impact.

dc.format.extent1018-1025
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherWiley
dc.subjectArabidopsis
dc.subjectconfocal microscopy
dc.subjectfluorescence
dc.subjectmesophyll
dc.subjectperfluorocarbon
dc.subjectperfluorodecalin
dc.titlePerfluorodecalin enhances <i>in vivo</i> confocal microscopy resolution of <i>Arabidopsis thaliana</i> mesophyll
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/20374500
plymouth.issue4
plymouth.volume186
plymouth.publisher-urlhttp://dx.doi.org/10.1111/j.1469-8137.2010.03244.x
plymouth.publication-statusPublished
plymouth.journalNew Phytologist
dc.identifier.doi10.1111/j.1469-8137.2010.03244.x
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeEngland
dc.identifier.eissn1469-8137
dc.rights.embargoperiodNo embargo
rioxxterms.versionofrecord10.1111/j.1469-8137.2010.03244.x
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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