Septin-Dependent Assembly of the Exocyst Is Essential for Plant Infection by <i>Magnaporthe oryzae</i>
dc.contributor.author | Gupta, Yogesh | |
dc.contributor.author | Dagdas, Yasin | |
dc.contributor.author | Martinez-Rocha, A-L | |
dc.contributor.author | Kershaw, MJ | |
dc.contributor.author | Littlejohn, George | |
dc.contributor.author | Ryder, Lauren | |
dc.contributor.author | Sklenar, Jan | |
dc.contributor.author | Delgado Rodriguez, Elena krisstell | |
dc.contributor.author | Nicholas, Talbot | |
dc.date.accessioned | 2017-05-24T19:07:22Z | |
dc.date.available | 2017-05-24T19:07:22Z | |
dc.date.issued | 2015-11 | |
dc.identifier.issn | 1040-4651 | |
dc.identifier.issn | 1532-298X | |
dc.identifier.uri | http://hdl.handle.net/10026.1/9337 | |
dc.description.abstract |
Magnaporthe oryzae is the causal agent of rice blast disease, the most devastating disease of cultivated rice (Oryza sativa) and a continuing threat to global food security. To cause disease, the fungus elaborates a specialized infection cell called an appressorium, which breaches the cuticle of the rice leaf, allowing the fungus entry to plant tissue. Here, we show that the exocyst complex localizes to the tips of growing hyphae during vegetative growth, ahead of the Spitzenkörper, and is required for polarized exocytosis. However, during infection-related development, the exocyst specifically assembles in the appressorium at the point of plant infection. The exocyst components Sec3, Sec5, Sec6, Sec8, and Sec15, and exocyst complex proteins Exo70 and Exo84 localize specifically in a ring formation at the appressorium pore. Targeted gene deletion, or conditional mutation, of genes encoding exocyst components leads to impaired plant infection. We demonstrate that organization of the exocyst complex at the appressorium pore is a septin-dependent process, which also requires regulated synthesis of reactive oxygen species by the NoxR-dependent Nox2 NADPH oxidase complex. We conclude that septin-mediated assembly of the exocyst is necessary for appressorium repolarization and host cell invasion. | |
dc.format.extent | 3277-3289 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press (OUP) | |
dc.subject | Fungal Proteins | |
dc.subject | Hyphae | |
dc.subject | Immunoprecipitation | |
dc.subject | Magnaporthe | |
dc.subject | Plant Diseases | |
dc.subject | Protein Subunits | |
dc.subject | Protein Transport | |
dc.subject | Reactive Oxygen Species | |
dc.subject | Septins | |
dc.subject | Spores, Fungal | |
dc.subject | Subcellular Fractions | |
dc.title | Septin-Dependent Assembly of the Exocyst Is Essential for Plant Infection by <i>Magnaporthe oryzae</i> | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000368295800019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 11 | |
plymouth.volume | 27 | |
plymouth.publication-status | Published | |
plymouth.journal | The Plant Cell | |
dc.identifier.doi | 10.1105/tpc.15.00552 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | England | |
dcterms.dateAccepted | 2015-10-20 | |
dc.identifier.eissn | 1532-298X | |
dc.rights.embargoperiod | No embargo | |
rioxxterms.versionofrecord | 10.1105/tpc.15.00552 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2015-11 | |
rioxxterms.type | Journal Article/Review |