Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers
dc.contributor.author | Pemberton, R | |
dc.contributor.author | Cox, T | |
dc.contributor.author | Tuffin, R | |
dc.contributor.author | Drago, G | |
dc.contributor.author | Griffiths, J | |
dc.contributor.author | Pittson, R | |
dc.contributor.author | Johnson, G | |
dc.contributor.author | Xu, J | |
dc.contributor.author | Sage, I | |
dc.contributor.author | Davies, R | |
dc.contributor.author | Jackson, S | |
dc.contributor.author | Kenna, G | |
dc.contributor.author | Luxton, R | |
dc.contributor.author | Hart, J | |
dc.date.accessioned | 2017-05-15T17:13:53Z | |
dc.date.available | 2017-05-15T17:13:53Z | |
dc.date.issued | 2014-11 | |
dc.identifier.issn | 1424-8220 | |
dc.identifier.issn | 1424-8220 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/9257 | |
dc.description.abstract |
This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies. | |
dc.format.extent | 20519-20532 | |
dc.format.medium | Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | MDPI AG | |
dc.subject | microbiosensor | |
dc.subject | screen-printing | |
dc.subject | MEMS | |
dc.subject | 96-well plate | |
dc.subject | metabolism | |
dc.subject | amperometry | |
dc.subject | cell monitoring | |
dc.subject | toxicity testing | |
dc.title | Fabrication and Evaluation of a Micro(Bio)Sensor Array Chip for Multiple Parallel Measurements of Important Cell Biomarkers | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000345562800034&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 11 | |
plymouth.volume | 14 | |
plymouth.publication-status | Published online | |
plymouth.journal | Sensors | |
dc.identifier.doi | 10.3390/s141120519 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | Switzerland | |
dcterms.dateAccepted | 2014-10-21 | |
dc.identifier.eissn | 1424-8220 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.3390/s141120519 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2014-10-30 | |
rioxxterms.type | Journal Article/Review |