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dc.contributor.authorKamhieh-Milz, Jen
dc.contributor.authorMoftah, RFHen
dc.contributor.authorBal, Gen
dc.contributor.authorFutschik, Men
dc.contributor.authorSterzer, Ven
dc.contributor.authorKhorramshahi, Oen
dc.contributor.authorBurow, Men
dc.contributor.authorThiel, Gen
dc.contributor.authorStuke-Sontheimer, Aen
dc.contributor.authorChaoui, Ren
dc.contributor.authorKamhieh-Milz, Sen
dc.contributor.authorSalama, Aen
dc.date.accessioned2017-02-07T19:47:10Z
dc.date.available2017-02-07T19:47:10Z
dc.date.issued2014en
dc.identifier.urihttp://hdl.handle.net/10026.1/8403
dc.description.abstract

OBJECTIVES: Most developmental processes are under the control of small regulatory RNAs called microRNAs (miRNAs). We hypothesize that different fetal developmental processes might be reflected by extracellular miRNAs in maternal plasma and may be utilized as biomarkers for the noninvasive prenatal diagnosis of chromosomal aneuploidies. In this proof-of-concept study, we report on the identification of extracellular miRNAs in maternal plasma of Down syndrome (DS) pregnancies. METHODS: Using high-throughput quantitative PCR (HT-qPCR), 1043 miRNAs were investigated in maternal plasma via comparison of seven DS pregnancies with age and fetal sex matched controls. RESULTS: Six hundred and ninety-five miRNAs were identified. Thirty-six significantly differentially expressed mature miRNAs were identified as potential biomarkers. Hierarchical cluster analysis of these miRNAs resulted in the clear discrimination of DS from euploid pregnancies. Gene targets of the differentially expressed miRNAs were enriched in signaling pathways such as mucin type-O-glycans, ECM-receptor interactions, TGF-beta, and endocytosis, which have been previously associated with DS. CONCLUSIONS: miRNAs are promising and stable biomarkers for a broad range of diseases and may allow a reliable, cost-efficient diagnostic tool for the noninvasive prenatal diagnosis of DS.

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dc.format.extent402475 - ?en
dc.languageengen
dc.language.isoengen
dc.subjectAdulten
dc.subjectBiomarkersen
dc.subjectDown Syndromeen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectMicroRNAsen
dc.subjectPregnancyen
dc.subjectPrenatal Diagnosisen
dc.subjectTransforming Growth Factor betaen
dc.subjectTrisomyen
dc.titleDifferentially expressed microRNAs in maternal plasma for the noninvasive prenatal diagnosis of Down syndrome (trisomy 21).en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/25478570en
plymouth.volume2014en
plymouth.publication-statusPublisheden
plymouth.journalBiomed Res Inten
dc.identifier.doi10.1155/2014/402475en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry
plymouth.organisational-group/Plymouth/Faculty of Medicine and Dentistry/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
dc.publisher.placeUnited Statesen
dcterms.dateAccepted2014-09-09en
dc.identifier.eissn2314-6141en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1155/2014/402475en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014en
rioxxterms.typeJournal Article/Reviewen


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