Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function.
dc.contributor.author | Worah, K | |
dc.contributor.author | Mathan, TS | |
dc.contributor.author | Vu Manh, TP | |
dc.contributor.author | Keerthikumar, S | |
dc.contributor.author | Schreibelt, G | |
dc.contributor.author | Tel, J | |
dc.contributor.author | Duiveman-de Boer, T | |
dc.contributor.author | Sköld, AE | |
dc.contributor.author | van Spriel, AB | |
dc.contributor.author | de Vries, IJ | |
dc.contributor.author | Huynen, MA | |
dc.contributor.author | Wessels, HJ | |
dc.contributor.author | Gloerich, J | |
dc.contributor.author | Dalod, M | |
dc.contributor.author | Lasonder, Edwin | |
dc.contributor.author | Figdor, CG | |
dc.contributor.author | Buschow, SI | |
dc.date.accessioned | 2016-09-15T12:05:52Z | |
dc.date.available | 2016-09-15T12:05:52Z | |
dc.date.issued | 2016-09-13 | |
dc.identifier.issn | 2211-1247 | |
dc.identifier.issn | 2211-1247 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/5448 | |
dc.description.abstract |
Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. Although RNA-expression profiles have been previously compared, protein expression data may provide a different picture. Here, we exploited label-free quantitative mass spectrometry to compare and identify differences in primary human DC subset proteins. Moreover, we integrated these proteomic data with existing mRNA data to derive robust cell-specific expression signatures with more than 400 differentially expressed proteins between subsets, forming a solid basis for investigation of subset-specific functions. We illustrated this by extracting subset identification markers and by demonstrating that pDCs lack caspase-1 and only express low levels of other inflammasome-related proteins. In accordance, pDCs were incapable of interleukin (IL)-1β secretion in response to ATP. | |
dc.format.extent | 2953-2966 | |
dc.format.medium | ||
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Elsevier BV | |
dc.subject | Biomarkers | |
dc.subject | Caspase 1 | |
dc.subject | Dendritic Cells | |
dc.subject | Gene Expression Profiling | |
dc.subject | Gene Expression Regulation | |
dc.subject | Humans | |
dc.subject | Inflammasomes | |
dc.subject | Protein Interaction Maps | |
dc.subject | Proteomics | |
dc.subject | Reproducibility of Results | |
dc.subject | Transcriptome | |
dc.title | Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function. | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | http://www.ncbi.nlm.nih.gov/pubmed/27626665 | |
plymouth.issue | 11 | |
plymouth.volume | 16 | |
plymouth.publication-status | Published | |
plymouth.journal | Cell Rep | |
dc.identifier.doi | 10.1016/j.celrep.2016.08.023 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2016-08-05 | |
dc.identifier.eissn | 2211-1247 | |
dc.rights.embargoperiod | No embargo | |
rioxxterms.versionofrecord | 10.1016/j.celrep.2016.08.023 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2016-09-13 | |
rioxxterms.type | Journal Article/Review | |
plymouth.oa-location | http://www.sciencedirect.com/science/article/pii/S2211124716310701?via=ihub |