Epstein-Barr virus-encoded microRNA BART1 induces tumour metastasis by regulating PTEN-dependent pathways in nasopharyngeal carcinoma.
dc.contributor.author | Cai, L | en |
dc.contributor.author | Ye, Y | en |
dc.contributor.author | Jiang, Q | en |
dc.contributor.author | Chen, Y | en |
dc.contributor.author | Lyu, X | en |
dc.contributor.author | Li, J | en |
dc.contributor.author | Wang, S | en |
dc.contributor.author | Liu, T | en |
dc.contributor.author | Cai, H | en |
dc.contributor.author | Yao, K | en |
dc.contributor.author | Li, J-L | en |
dc.contributor.author | Li, X | en |
dc.date.accessioned | 2016-08-09T14:48:42Z | |
dc.date.available | 2016-08-09T14:48:42Z | |
dc.date.issued | 2015-07-02 | en |
dc.identifier.uri | http://hdl.handle.net/10026.1/5246 | |
dc.description.abstract |
Epstein-Barr virus (EBV), aetiologically linked to nasopharyngeal carcinoma (NPC), is the first human virus found to encode many miRNAs. However, how these viral miRNAs precisely regulate the tumour metastasis in NPC remains obscure. Here we report that EBV-miR-BART1 is highly expressed in NPC and closely associated with pathological and advanced clinical stages of NPC. Alteration of EBV-miR-BART1 expression results in an increase in migration and invasion of NPC cells in vitro and causes tumour metastasis in vivo. Mechanistically, EBV-miR-BART1 directly targets the cellular tumour suppressor PTEN. Reduction of PTEN dosage by EBV-miR-BART1 activates PTEN-dependent pathways including PI3K-Akt, FAK-p130(Cas) and Shc-MAPK/ERK1/2 signalling, drives EMT, and consequently increases migration, invasion and metastasis of NPC cells. Reconstitution of PTEN rescues all phenotypes generated by EBV-miR-BART1, highlighting the role of PTEN in EBV-miR-BART-driven metastasis in NPC. Our findings provide new insights into the metastasis of NPC regulated by EBV and advocate for developing clinical intervention strategies against NPC. | en |
dc.format.extent | 7353 - ? | en |
dc.language | eng | en |
dc.language.iso | eng | en |
dc.subject | Adult | en |
dc.subject | Animals | en |
dc.subject | Blotting, Western | en |
dc.subject | Carcinoma | en |
dc.subject | Carcinoma, Squamous Cell | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Female | en |
dc.subject | Gene Expression Regulation, Neoplastic | en |
dc.subject | HEK293 Cells | en |
dc.subject | Head and Neck Neoplasms | en |
dc.subject | Herpesvirus 4, Human | en |
dc.subject | Humans | en |
dc.subject | Immunohistochemistry | en |
dc.subject | Male | en |
dc.subject | Mice | en |
dc.subject | Mice, Nude | en |
dc.subject | MicroRNAs | en |
dc.subject | Nasopharyngeal Carcinoma | en |
dc.subject | Nasopharyngeal Neoplasms | en |
dc.subject | Neoplasm Metastasis | en |
dc.subject | Neoplasm Transplantation | en |
dc.subject | PTEN Phosphohydrolase | en |
dc.subject | RNA, Viral | en |
dc.subject | Real-Time Polymerase Chain Reaction | en |
dc.subject | Signal Transduction | en |
dc.subject | Squamous Cell Carcinoma of Head and Neck | en |
dc.title | Epstein-Barr virus-encoded microRNA BART1 induces tumour metastasis by regulating PTEN-dependent pathways in nasopharyngeal carcinoma. | en |
dc.type | Journal Article | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/26135619 | en |
plymouth.volume | 6 | en |
plymouth.publication-status | Published online | en |
plymouth.journal | Nat Commun | en |
dc.identifier.doi | 10.1038/ncomms8353 | en |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine | |
dc.publisher.place | England | en |
dcterms.dateAccepted | 2015-04-29 | en |
dc.identifier.eissn | 2041-1723 | en |
dc.rights.embargoperiod | Not known | en |
rioxxterms.versionofrecord | 10.1038/ncomms8353 | en |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | en |
rioxxterms.licenseref.startdate | 2015-07-02 | en |
rioxxterms.type | Journal Article/Review | en |