Experimentally controlled downregulation of the histone chaperone FACT in<i>Plasmodium berghei</i>reveals that it is critical to male gamete fertility
dc.contributor.author | Laurentino, EC | |
dc.contributor.author | Taylor, S | |
dc.contributor.author | Mair, GR | |
dc.contributor.author | Lasonder, Edwin | |
dc.contributor.author | Bartfai, R | |
dc.contributor.author | Stunnenberg, HG | |
dc.contributor.author | Kroeze, H | |
dc.contributor.author | Ramesar, J | |
dc.contributor.author | Franke‐Fayard, B | |
dc.contributor.author | Khan, SM | |
dc.contributor.author | Janse, CJ | |
dc.contributor.author | Waters, AP | |
dc.date.accessioned | 2016-07-11T15:05:20Z | |
dc.date.available | 2016-07-11T15:05:20Z | |
dc.date.issued | 2011-12 | |
dc.identifier.issn | 1462-5814 | |
dc.identifier.issn | 1462-5822 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/5046 | |
dc.description.abstract |
Human FACT (facilitates chromatin transcription) consists of the proteins SPT16 and SSRP1 and acts as a histone chaperone in the (dis)assembly of nucleosome (and thereby chromatin) structure during transcription and DNA replication. We identified a Plasmodium berghei protein, termed FACT-L, with homology to the SPT16 subunit of FACT. Epitope tagging of FACT-L showed nuclear localization with high expression in the nuclei of (activated) male gametocytes. The gene encoding FACT-L could not be deleted indicating an essential role during blood-stage development. Using a 'promoter-swap' approach whereby the fact-l promoter was replaced by an 'asexual blood stage-specific' promoter that is silent in gametocytes, transcription of fact-l in promoter-swap mutant gametocytes was downregulated compared with wild-type gametocytes. These mutant male gametocytes showed delayed DNA replication and gamete formation. Male gamete fertility was strongly reduced while female gamete fertility was unaffected; residual ookinetes generated oocysts that arrested early in development and failed to enter sporogony. Therefore FACT is critically involved in the formation of fertile male gametes and parasite transmission. 'Promoter swapping' is a powerful approach for the functional analysis of proteins in gametocytes (and beyond) that are essential during asexual blood-stage development. | |
dc.format.extent | 1956-1974 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Hindawi Limited | |
dc.subject | Animals | |
dc.subject | Anopheles | |
dc.subject | Cell Nucleus | |
dc.subject | DNA Replication | |
dc.subject | DNA, Protozoan | |
dc.subject | DNA-Binding Proteins | |
dc.subject | Down-Regulation | |
dc.subject | Epitope Mapping | |
dc.subject | Female | |
dc.subject | Fertility | |
dc.subject | Flagella | |
dc.subject | Gene Expression Regulation | |
dc.subject | Gene Knockdown Techniques | |
dc.subject | Genetic Vectors | |
dc.subject | Germ Cells | |
dc.subject | Histone Chaperones | |
dc.subject | Mice | |
dc.subject | Oocysts | |
dc.subject | Plasmodium berghei | |
dc.subject | Promoter Regions, Genetic | |
dc.subject | Protein Biosynthesis | |
dc.subject | Protozoan Proteins | |
dc.subject | Transcription, Genetic | |
dc.title | Experimentally controlled downregulation of the histone chaperone FACT in<i>Plasmodium berghei</i>reveals that it is critical to male gamete fertility | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000297115000011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 12 | |
plymouth.volume | 13 | |
plymouth.publication-status | Published | |
plymouth.journal | Cellular Microbiology | |
dc.identifier.doi | 10.1111/j.1462-5822.2011.01683.x | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
dc.publisher.place | India | |
dc.identifier.eissn | 1462-5822 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1111/j.1462-5822.2011.01683.x | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review |