Proteome Based Construction of the Lymphocyte Function-Associated Antigen 1 (LFA-1) Interactome in Human Dendritic Cells
dc.contributor.author | Eich, C | |
dc.contributor.author | Lasonder, Edwin | |
dc.contributor.author | Cruz, LJ | |
dc.contributor.author | Reinieren-Beeren, I | |
dc.contributor.author | Cambi, A | |
dc.contributor.author | Figdor, CG | |
dc.contributor.author | Buschow, SI | |
dc.date.accessioned | 2016-07-11T15:03:22Z | |
dc.date.available | 2016-07-11T15:03:22Z | |
dc.date.issued | 2016-02-18 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | ARTN e0149637 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/5042 | |
dc.description.abstract |
The β2-integrin lymphocyte function-associated antigen 1 (LFA-1) plays an important role in the migration, adhesion and intercellular communication of dendritic cells (DCs). During the differentiation of human DCs from monocyte precursors, LFA-1 ligand binding capacity is completely lost, even though its expression levels were remained constant. Yet LFA-1-mediated adhesive capacity on DCs can be regained by exposing DCs to the chemokine CCL21, suggesting a high degree of regulation of LFA-1 activity during the course of DC differentiation. The molecular mechanisms underlying this regulation of LFA-1 function in DCs, however, remain elusive. To get more insight we attempted to identify specific LFA-1 binding partners that may play a role in regulating LFA-1 activity in DCs. We used highly sensitive label free quantitative mass-spectrometry to identify proteins co-immunoprecipitated (co-IP) with LFA-1 from ex vivo generated DCs. Among the potential binding partners we identified not only established components of integrin signalling pathways and cytoskeletal proteins, but also several novel LFA-1 binding partners including CD13, galectin-3, thrombospondin-1 and CD44. Further comparison to the LFA-1 interaction partners in monocytes indicated that DC differentiation was accompanied by an overall increase in LFA-1 associated proteins, in particular cytoskeletal, signalling and plasma membrane (PM) proteins. The here presented LFA-1 interactome composed of 78 proteins thus represents a valuable resource of potential regulators of LFA-1 function during the DC lifecycle. | |
dc.format.extent | e0149637-e0149637 | |
dc.format.medium | Electronic-eCollection | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Public Library of Science (PLoS) | |
dc.subject | Blotting, Western | |
dc.subject | CD13 Antigens | |
dc.subject | Cell Membrane | |
dc.subject | Computer Simulation | |
dc.subject | Dendritic Cells | |
dc.subject | Galectin 3 | |
dc.subject | Humans | |
dc.subject | Hyaluronan Receptors | |
dc.subject | Immunoprecipitation | |
dc.subject | Integrins | |
dc.subject | Ligands | |
dc.subject | Lymphocyte Function-Associated Antigen-1 | |
dc.subject | Mass Spectrometry | |
dc.subject | Monocytes | |
dc.subject | Protein Binding | |
dc.subject | Protein Interaction Maps | |
dc.subject | Proteome | |
dc.subject | Reproducibility of Results | |
dc.title | Proteome Based Construction of the Lymphocyte Function-Associated Antigen 1 (LFA-1) Interactome in Human Dendritic Cells | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000371221500065&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 2 | |
plymouth.volume | 11 | |
plymouth.publication-status | Published online | |
plymouth.journal | PLOS ONE | |
dc.identifier.doi | 10.1371/journal.pone.0149637 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2016-02-03 | |
dc.identifier.eissn | 1932-6203 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1371/journal.pone.0149637 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2016 | |
rioxxterms.type | Journal Article/Review |