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dc.contributor.authorPetty, NKen
dc.contributor.authorBen Zakour, NLen
dc.contributor.authorStanton-Cook, Men
dc.contributor.authorSkippington, Een
dc.contributor.authorTotsika, Men
dc.contributor.authorForde, BMen
dc.contributor.authorPhan, M-Den
dc.contributor.authorGomes Moriel, Den
dc.contributor.authorPeters, KMen
dc.contributor.authorDavies, Men
dc.contributor.authorRogers, BAen
dc.contributor.authorDougan, Gen
dc.contributor.authorRodriguez-Baño, Jen
dc.contributor.authorPascual, Aen
dc.contributor.authorPitout, JDDen
dc.contributor.authorUpton, Men
dc.contributor.authorPaterson, DLen
dc.contributor.authorWalsh, TRen
dc.contributor.authorSchembri, MAen
dc.contributor.authorBeatson, SAen

Escherichia coli sequence type 131 (ST131) is a globally disseminated, multidrug resistant (MDR) clone responsible for a high proportion of urinary tract and bloodstream infections. The rapid emergence and successful spread of E. coli ST131 is strongly associated with several factors, including resistance to fluoroquinolones, high virulence gene content, the possession of the type 1 fimbriae FimH30 allele, and the production of the CTX-M-15 extended spectrum β-lactamase (ESBL). Here, we used genome sequencing to examine the molecular epidemiology of a collection of E. coli ST131 strains isolated from six distinct geographical locations across the world spanning 2000-2011. The global phylogeny of E. coli ST131, determined from whole-genome sequence data, revealed a single lineage of E. coli ST131 distinct from other extraintestinal E. coli strains within the B2 phylogroup. Three closely related E. coli ST131 sublineages were identified, with little association to geographic origin. The majority of single-nucleotide variants associated with each of the sublineages were due to recombination in regions adjacent to mobile genetic elements (MGEs). The most prevalent sublineage of ST131 strains was characterized by fluoroquinolone resistance, and a distinct virulence factor and MGE profile. Four different variants of the CTX-M ESBL-resistance gene were identified in our ST131 strains, with acquisition of CTX-M-15 representing a defining feature of a discrete but geographically dispersed ST131 sublineage. This study confirms the global dispersal of a single E. coli ST131 clone and demonstrates the role of MGEs and recombination in the evolution of this important MDR pathogen.

dc.format.extent5694 - 5699en
dc.subjectbacterial evolutionen
dc.subjectgenomic epidemiologyen
dc.subjectBase Sequenceen
dc.subjectComputational Biologyen
dc.subjectDrug Resistance, Multiple, Bacterialen
dc.subjectEscherichia colien
dc.subjectGenome, Bacterialen
dc.subjectLikelihood Functionsen
dc.subjectModels, Geneticen
dc.subjectMolecular Sequence Dataen
dc.subjectPolymorphism, Single Nucleotideen
dc.subjectSequence Alignmenten
dc.subjectSequence Analysis, DNAen
dc.subjectSpecies Specificityen
dc.titleGlobal dissemination of a multidrug resistant Escherichia coli clone.en
dc.typeJournal Article
plymouth.journalProc Natl Acad Sci U S Aen
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited Statesen
dc.rights.embargoperiodNo embargoen
rioxxterms.typeJournal Article/Reviewen

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