Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms
dc.contributor.author | Totsika, M | |
dc.contributor.author | Beatson, SA | |
dc.contributor.author | Sarkar, S | |
dc.contributor.author | Phan, M-D | |
dc.contributor.author | Petty, NK | |
dc.contributor.author | Bachmann, N | |
dc.contributor.author | Szubert, M | |
dc.contributor.author | Sidjabat, HE | |
dc.contributor.author | Paterson, DL | |
dc.contributor.author | Upton, Mathew | |
dc.contributor.author | Schembri, MA | |
dc.date.accessioned | 2016-04-18T20:13:40Z | |
dc.date.available | 2016-04-18T20:13:40Z | |
dc.date.issued | 2011-10-28 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | ARTN e26578 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/4516 | |
dc.description.abstract |
Escherichia coli strains causing urinary tract infection (UTI) are increasingly recognized as belonging to specific clones. E. coli clone O25b:H4-ST131 has recently emerged globally as a leading multi-drug resistant pathogen causing urinary tract and bloodstream infections in hospitals and the community. While most molecular studies to date examine the mechanisms conferring multi-drug resistance in E. coli ST131, relatively little is known about their virulence potential. Here we examined E. coli ST131 clinical isolates from two geographically diverse collections, one representing the major pathogenic lineages causing UTI across the United Kingdom and a second representing UTI isolates from patients presenting at two large hospitals in Australia. We determined a draft genome sequence for one representative isolate, E. coli EC958, which produced CTX-M-15 extended-spectrum β-lactamase, CMY-23 type AmpC cephalosporinase and was resistant to ciprofloxacin. Comparative genome analysis indicated that EC958 encodes virulence genes commonly associated with uropathogenic E. coli (UPEC). The genome sequence of EC958 revealed a transposon insertion in the fimB gene encoding the activator of type 1 fimbriae, an important UPEC bladder colonization factor. We identified the same fimB transposon insertion in 59% of the ST131 UK isolates, as well as 71% of ST131 isolates from Australia, suggesting this mutation is common among E. coli ST131 strains. Insertional inactivation of fimB resulted in a phenotype resembling a slower off-to-on switching for type 1 fimbriae. Type 1 fimbriae expression could still be induced in fimB-null isolates; this correlated strongly with adherence to and invasion of human bladder cells and bladder colonisation in a mouse UTI model. We conclude that E. coli ST131 is a geographically widespread, antibiotic resistant clone that has the capacity to produce numerous virulence factors associated with UTI. | |
dc.format.extent | e26578-e26578 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Public Library of Science (PLoS) | |
dc.subject | Animals | |
dc.subject | Anti-Bacterial Agents | |
dc.subject | Australia | |
dc.subject | Base Sequence | |
dc.subject | Colony Count, Microbial | |
dc.subject | Disease Models, Animal | |
dc.subject | Drug Resistance, Multiple, Bacterial | |
dc.subject | Escherichia coli | |
dc.subject | Escherichia coli Infections | |
dc.subject | Fimbriae, Bacterial | |
dc.subject | Genes, Bacterial | |
dc.subject | Genome, Bacterial | |
dc.subject | Humans | |
dc.subject | Internationality | |
dc.subject | Mice | |
dc.subject | Mutagenesis, Insertional | |
dc.subject | Mutation | |
dc.subject | Operon | |
dc.subject | Phylogeny | |
dc.subject | Plasmids | |
dc.subject | Sequence Analysis, DNA | |
dc.subject | United Kingdom | |
dc.subject | Urinary Bladder | |
dc.subject | Urine | |
dc.subject | Virulence | |
dc.subject | Virulence Factors | |
dc.title | Insights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000299081800023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 10 | |
plymouth.volume | 6 | |
plymouth.publication-status | Published online | |
plymouth.journal | PLoS ONE | |
dc.identifier.doi | 10.1371/journal.pone.0026578 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR) | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2011-09-29 | |
dc.identifier.eissn | 1932-6203 | |
dc.rights.embargoperiod | No embargo | |
rioxxterms.versionofrecord | 10.1371/journal.pone.0026578 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2011 | |
rioxxterms.type | Journal Article/Review |