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dc.contributor.authorTotsika, M
dc.contributor.authorBeatson, SA
dc.contributor.authorSarkar, S
dc.contributor.authorPhan, M-D
dc.contributor.authorPetty, NK
dc.contributor.authorBachmann, N
dc.contributor.authorSzubert, M
dc.contributor.authorSidjabat, HE
dc.contributor.authorPaterson, DL
dc.contributor.authorUpton, Mathew
dc.contributor.authorSchembri, MA
dc.date.accessioned2016-04-18T20:13:40Z
dc.date.available2016-04-18T20:13:40Z
dc.date.issued2011-10-28
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.otherARTN e26578
dc.identifier.urihttp://hdl.handle.net/10026.1/4516
dc.description.abstract

Escherichia coli strains causing urinary tract infection (UTI) are increasingly recognized as belonging to specific clones. E. coli clone O25b:H4-ST131 has recently emerged globally as a leading multi-drug resistant pathogen causing urinary tract and bloodstream infections in hospitals and the community. While most molecular studies to date examine the mechanisms conferring multi-drug resistance in E. coli ST131, relatively little is known about their virulence potential. Here we examined E. coli ST131 clinical isolates from two geographically diverse collections, one representing the major pathogenic lineages causing UTI across the United Kingdom and a second representing UTI isolates from patients presenting at two large hospitals in Australia. We determined a draft genome sequence for one representative isolate, E. coli EC958, which produced CTX-M-15 extended-spectrum β-lactamase, CMY-23 type AmpC cephalosporinase and was resistant to ciprofloxacin. Comparative genome analysis indicated that EC958 encodes virulence genes commonly associated with uropathogenic E. coli (UPEC). The genome sequence of EC958 revealed a transposon insertion in the fimB gene encoding the activator of type 1 fimbriae, an important UPEC bladder colonization factor. We identified the same fimB transposon insertion in 59% of the ST131 UK isolates, as well as 71% of ST131 isolates from Australia, suggesting this mutation is common among E. coli ST131 strains. Insertional inactivation of fimB resulted in a phenotype resembling a slower off-to-on switching for type 1 fimbriae. Type 1 fimbriae expression could still be induced in fimB-null isolates; this correlated strongly with adherence to and invasion of human bladder cells and bladder colonisation in a mouse UTI model. We conclude that E. coli ST131 is a geographically widespread, antibiotic resistant clone that has the capacity to produce numerous virulence factors associated with UTI.

dc.format.extente26578-e26578
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.subjectAnimals
dc.subjectAnti-Bacterial Agents
dc.subjectAustralia
dc.subjectBase Sequence
dc.subjectColony Count, Microbial
dc.subjectDisease Models, Animal
dc.subjectDrug Resistance, Multiple, Bacterial
dc.subjectEscherichia coli
dc.subjectEscherichia coli Infections
dc.subjectFimbriae, Bacterial
dc.subjectGenes, Bacterial
dc.subjectGenome, Bacterial
dc.subjectHumans
dc.subjectInternationality
dc.subjectMice
dc.subjectMutagenesis, Insertional
dc.subjectMutation
dc.subjectOperon
dc.subjectPhylogeny
dc.subjectPlasmids
dc.subjectSequence Analysis, DNA
dc.subjectUnited Kingdom
dc.subjectUrinary Bladder
dc.subjectUrine
dc.subjectVirulence
dc.subjectVirulence Factors
dc.titleInsights into a Multidrug Resistant Escherichia coli Pathogen of the Globally Disseminated ST131 Lineage: Genome Analysis and Virulence Mechanisms
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000299081800023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue10
plymouth.volume6
plymouth.publication-statusPublished online
plymouth.journalPLoS ONE
dc.identifier.doi10.1371/journal.pone.0026578
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR)
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
plymouth.organisational-group/Plymouth/Users by role/Researchers in ResearchFish submission
dc.publisher.placeUnited States
dcterms.dateAccepted2011-09-29
dc.identifier.eissn1932-6203
dc.rights.embargoperiodNo embargo
rioxxterms.versionofrecord10.1371/journal.pone.0026578
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2011
rioxxterms.typeJournal Article/Review


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