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dc.contributor.authorPark, J
dc.contributor.authorYoon, J-H
dc.contributor.authorDepuydt, S
dc.contributor.authorOh, J-W
dc.contributor.authorJo, Y-M
dc.contributor.authorKim, K
dc.contributor.authorBrown, Murray
dc.contributor.authorHan, T
dc.date.accessioned2016-04-13T14:24:51Z
dc.date.available2016-04-13T14:24:51Z
dc.date.issued2016-04-01
dc.identifier.issn0147-6513
dc.identifier.issn1090-2414
dc.identifier.urihttp://hdl.handle.net/10026.1/4492
dc.description.abstract

The root elongation bioassay is one of the most straightforward test methods used for environmental monitoring in terms of simplicity, rapidity and economy since it merely requires filter paper, distilled water and Petri dishes. However, filter paper as a support material is known to be problematic as it can reduce the sensitivity of the test.

The newly developed hydroponic method reported here differs from the conventional root elongation method (US EPA filter paper method) in that no support material is used and the exposure time is shorter (48 h in this test versus 120 h in the US EPA test). For metals, the hydroponic test method was 3.3 (for Hg) to 57 (for Cu) times more sensitive than the US EPA method with the rank orders of sensitivity, estimated from EC50 values, being Cu≥Cd>Ni≥Zn≥Hg for the former and Hg≥Cu≥Ni≥Cd≥Zn for the latter methods. For phenol, the results did not differ significantly; EC50 values were 124 mg L−1 and 108–180 mg L−1 for the hydroponic and filter paper methods, respectively. Lettuce was less sensitive than daphnids to wastewaters, but the root elongation response appears to be wastewater-specific and is especially sensitive for detecting the presence of fluorine.

The new hydroponic test thus provides many practical advantages, especially in terms of cost and time-effectiveness requiring only a well plate, a small volume of distilled water and short exposure period; furthermore, no specialist expertise is required. The method is simpler than the conventional EPA technique in not using filter paper which can influence the sensitivity of the test. Additionally, plant seeds have a long shelf-life and require little or no maintenance.

dc.format.extent147-153
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherElsevier BV
dc.subjectDaphnia
dc.subjectLettuce
dc.subjectMetals
dc.subjectPhenol
dc.subjectRoot elongation
dc.subjectWastewater
dc.titleThe sensitivity of an hydroponic lettuce root elongation bioassay to metals, phenol and wastewaters
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000370393700018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.volume126
plymouth.publication-statusPublished
plymouth.journalECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
dc.identifier.doi10.1016/j.ecoenv.2015.12.013
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Marine Institute
dc.publisher.placeNetherlands
dcterms.dateAccepted2015-12-10
dc.rights.embargodate2016-12-31
dc.identifier.eissn1090-2414
dc.rights.embargoperiod24 months
rioxxterms.versionofrecord10.1016/j.ecoenv.2015.12.013
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved
rioxxterms.licenseref.startdate2016-04-01
rioxxterms.typeJournal Article/Review
plymouth.oa-locationhttp://ac.els-cdn.com/S0147651315302001/1-s2.0-S0147651315302001-main.pdf?_tid=f6d50568-0182-11e6-9e0a-00000aab0f6c&acdnat=1460557448_b65f72d1f6a4d84d71537df2f5eef20b


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