Lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism
dc.contributor.author | Falcinelli, S | |
dc.contributor.author | Picchietti, S | |
dc.contributor.author | Rodiles, A | |
dc.contributor.author | Cossignani, L | |
dc.contributor.author | Merrifield, Daniel | |
dc.contributor.author | Taddei, AR | |
dc.contributor.author | Maradonna, F | |
dc.contributor.author | Olivotto, I | |
dc.contributor.author | Gioacchini, G | |
dc.contributor.author | Carnevali, O | |
dc.date.accessioned | 2015-08-13T21:53:52Z | |
dc.date.available | 2015-08-13T21:53:52Z | |
dc.date.issued | 2015-03-30 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | 9336 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/3520 | |
dc.description.abstract |
<jats:title>Abstract</jats:title><jats:p>The microbiome plays an important role in lipid metabolism but how the introduction of probiotic communities affects host lipid metabolism is poorly understood. Using a multidisciplinary approach we addressed this knowledge gap using the zebrafish model by coupling high-throughput sequencing with biochemical, molecular and morphological analysis to evaluate the changes in the intestine. Analysis of bacterial 16S libraries revealed that <jats:italic>Lactobacillus rhamnosus</jats:italic> was able to modulate the gut microbiome of zebrafish larvae, elevating the abundance of Firmicutes sequences and reducing the abundance of Actinobacteria. The gut microbiome changes modulated host lipid processing by inducing transcriptional down-regulation of genes involved in cholesterol and triglycerides metabolism (<jats:italic>fit2, agpat4, dgat2, mgll</jats:italic>, <jats:italic>hnf4α, scap</jats:italic> and <jats:italic>cck</jats:italic>) concomitantly decreasing total body cholesterol and triglyceride content and increasing fatty acid levels. <jats:italic>L. rhamnosus</jats:italic> treatment also increased microvilli and enterocyte lengths and decreased lipid droplet size in the intestinal epithelium. These changes resulted in elevated zebrafish larval growth. This integrated system investigation demonstrates probiotic modulation of the gut microbiome, highlights a novel gene network involved in lipid metabolism, provides an insight into how the microbiome regulates molecules involved in lipid metabolism and reveals a new potential role for <jats:italic>L. rhamnosus</jats:italic> in the treatment of lipid disorders.</jats:p> | |
dc.format.extent | 9336- | |
dc.format.medium | Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Springer Science and Business Media LLC | |
dc.subject | Animals | |
dc.subject | Biodiversity | |
dc.subject | Cholesterol | |
dc.subject | Gastrointestinal Microbiome | |
dc.subject | Gene Expression Regulation | |
dc.subject | Intestinal Mucosa | |
dc.subject | Lacticaseibacillus rhamnosus | |
dc.subject | Lipid Metabolism | |
dc.subject | Metagenome | |
dc.subject | Probiotics | |
dc.subject | Transcription, Genetic | |
dc.subject | Triglycerides | |
dc.subject | Zebrafish | |
dc.title | Lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000351935700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 1 | |
plymouth.volume | 5 | |
plymouth.publication-status | Published online | |
plymouth.journal | Scientific Reports | |
dc.identifier.doi | 10.1038/srep09336 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | England | |
dcterms.dateAccepted | 2015-02-09 | |
dc.identifier.eissn | 2045-2322 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1038/srep09336 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2015-03-30 | |
rioxxterms.type | Journal Article/Review |