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dc.contributor.authorFalcinelli, Sen
dc.contributor.authorPicchietti, Sen
dc.contributor.authorRodiles, Aen
dc.contributor.authorCossignani, Len
dc.contributor.authorMerrifield, DLen
dc.contributor.authorTaddei, ARen
dc.contributor.authorMaradonna, Fen
dc.contributor.authorOlivotto, Ien
dc.contributor.authorGioacchini, Gen
dc.contributor.authorCarnevali, Oen
dc.date.accessioned2015-08-13T21:53:52Z
dc.date.available2015-08-13T21:53:52Z
dc.date.issued2015-03-30en
dc.identifier.urihttp://hdl.handle.net/10026.1/3520
dc.description.abstract

The microbiome plays an important role in lipid metabolism but how the introduction of probiotic communities affects host lipid metabolism is poorly understood. Using a multidisciplinary approach we addressed this knowledge gap using the zebrafish model by coupling high-throughput sequencing with biochemical, molecular and morphological analysis to evaluate the changes in the intestine. Analysis of bacterial 16S libraries revealed that Lactobacillus rhamnosus was able to modulate the gut microbiome of zebrafish larvae, elevating the abundance of Firmicutes sequences and reducing the abundance of Actinobacteria. The gut microbiome changes modulated host lipid processing by inducing transcriptional down-regulation of genes involved in cholesterol and triglycerides metabolism (fit2, agpat4, dgat2, mgll, hnf4α, scap, and cck) concomitantly decreasing total body cholesterol and triglyceride content and increasing fatty acid levels. L. rhamnosus treatment also increased microvilli and enterocyte lengths and decreased lipid droplet size in the intestinal epithelium. These changes resulted in elevated zebrafish larval growth. This integrated system investigation demonstrates probiotic modulation of the gut microbiome, highlights a novel gene network involved in lipid metabolism, provides an insight into how the microbiome regulates molecules involved in lipid metabolism, and reveals a new potential role for L. rhamnosus in the treatment of lipid disorders.

en
dc.format.extent9336 - ?en
dc.languageengen
dc.language.isoengen
dc.subjectAnimalsen
dc.subjectBiodiversityen
dc.subjectCholesterolen
dc.subjectGastrointestinal Microbiomeen
dc.subjectGene Expression Regulationen
dc.subjectIntestinal Mucosaen
dc.subjectLactobacillus rhamnosusen
dc.subjectLipid Metabolismen
dc.subjectMetagenomeen
dc.subjectProbioticsen
dc.subjectTranscription, Geneticen
dc.subjectTriglyceridesen
dc.subjectZebrafishen
dc.titleLactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/25822072en
plymouth.volume5en
plymouth.publication-statusPublished onlineen
plymouth.journalSci Repen
dc.identifier.doi10.1038/srep09336en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 All current users
plymouth.organisational-group/Plymouth/00 All current users/Academics
plymouth.organisational-group/Plymouth/00 All current users/Academics/Faculty of Science & Engineering
plymouth.organisational-group/Plymouth/00 All current users/Academics/Faculty of Science & Engineering/School of Biological & Marine Sciences
plymouth.organisational-group/Plymouth/Faculty of Science & Engineering
plymouth.organisational-group/Plymouth/Faculty of Science & Engineering/School of Biological & Marine Sciences
dc.publisher.placeEnglanden_US
dc.publisher.placeEnglanden
dc.publisher.placeEnglanden
dc.publisher.placeEnglanden
dc.publisher.placeEnglanden
dc.publisher.placeEnglanden
dc.publisher.placeEnglanden
dc.publisher.placeEnglanden
dc.publisher.placeEnglanden
dcterms.dateAccepted2015-02-09en
dc.identifier.eissn2045-2322en
rioxxterms.versionofrecord10.1038/srep09336en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-03-30en
rioxxterms.typeJournal Article/Reviewen


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