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dc.contributor.authorHegde, NR
dc.contributor.authorDunn, C
dc.contributor.authorLewinsohn, DM
dc.contributor.authorJarvis, Michael A
dc.contributor.authorNelson, JA
dc.contributor.authorJohnson, DC
dc.date.accessioned2015-07-17T08:00:22Z
dc.date.available2015-07-17T08:00:22Z
dc.date.issued2005
dc.identifier.issn0022-1007
dc.identifier.issn1540-9538
dc.identifier.urihttp://hdl.handle.net/10026.1/3438
dc.descriptionTimes Cited: 43
dc.description.abstract

<jats:p>Human cytomegalovirus (HCMV) infects endothelial, epithelial, and glial cells in vivo. These cells can express MHC class II proteins, but are unlikely to play important roles in priming host immunity. Instead, it seems that class II presentation of endogenous HCMV antigens in these cells allows recognition of virus infection. We characterized class II presentation of HCMV glycoprotein B (gB), a membrane protein that accumulates extensively in endosomes during virus assembly. Human CD4+ T cells specific for gB were both highly abundant in blood and cytolytic in vivo. gB-specific CD4+ T cell clones recognized gB that was expressed in glial, endothelial, and epithelial cells, but not exogenous gB that was fed to these cells. Glial cells efficiently presented extremely low levels of endogenous gB—expressed by adenovirus vectors or after HCMV infection—and stimulated CD4+ T cells better than DCs that were incubated with exogenous gB. Presentation of endogenous gB required sorting of gB to endosomal compartments and processing by acidic proteases. Although presentation of cellular proteins that traffic into endosomes is well known, our observations demonstrate for the first time that a viral protein sorted to endosomes is presented exceptionally well, and can promote CD4+ T cell recognition and killing of biologically important host cells.</jats:p>

dc.format.extent1109-1119
dc.format.medium8
dc.languageen
dc.language.isoen
dc.publisherRockefeller University Press
dc.subjectAntibodies, Monoclonal
dc.subjectAntigen Presentation
dc.subjectCD4-Positive T-Lymphocytes
dc.subjectCell Line
dc.subjectEndocytosis
dc.subjectFlow Cytometry
dc.subjectHistocompatibility Antigens Class II
dc.subjectHumans
dc.subjectLymphocyte Subsets
dc.subjectMicroscopy, Fluorescence
dc.subjectNeuroglia
dc.subjectT-Lymphocytes, Cytotoxic
dc.subjectViral Envelope Proteins
dc.titleEndogenous human cytomegalovirus gB is presented efficiently by MHC class II molecules to CD4(+) CTL
dc.typejournal-article
dc.typeJOUR
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000232929500011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue8
plymouth.volume202
plymouth.publication-statusPublished
plymouth.journalJournal of Experimental Medicine
dc.identifier.doi10.1084/jem.20050162
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dc.identifier.eissn1540-9538
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1084/jem.20050162
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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