Endogenous human cytomegalovirus gB is presented efficiently by MHC class II molecules to CD4(+) CTL
dc.contributor.author | Hegde, NR | |
dc.contributor.author | Dunn, C | |
dc.contributor.author | Lewinsohn, DM | |
dc.contributor.author | Jarvis, Michael A | |
dc.contributor.author | Nelson, JA | |
dc.contributor.author | Johnson, DC | |
dc.date.accessioned | 2015-07-17T08:00:22Z | |
dc.date.available | 2015-07-17T08:00:22Z | |
dc.date.issued | 2005 | |
dc.identifier.issn | 0022-1007 | |
dc.identifier.issn | 1540-9538 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/3438 | |
dc.description | Times Cited: 43 | |
dc.description.abstract |
<jats:p>Human cytomegalovirus (HCMV) infects endothelial, epithelial, and glial cells in vivo. These cells can express MHC class II proteins, but are unlikely to play important roles in priming host immunity. Instead, it seems that class II presentation of endogenous HCMV antigens in these cells allows recognition of virus infection. We characterized class II presentation of HCMV glycoprotein B (gB), a membrane protein that accumulates extensively in endosomes during virus assembly. Human CD4+ T cells specific for gB were both highly abundant in blood and cytolytic in vivo. gB-specific CD4+ T cell clones recognized gB that was expressed in glial, endothelial, and epithelial cells, but not exogenous gB that was fed to these cells. Glial cells efficiently presented extremely low levels of endogenous gB—expressed by adenovirus vectors or after HCMV infection—and stimulated CD4+ T cells better than DCs that were incubated with exogenous gB. Presentation of endogenous gB required sorting of gB to endosomal compartments and processing by acidic proteases. Although presentation of cellular proteins that traffic into endosomes is well known, our observations demonstrate for the first time that a viral protein sorted to endosomes is presented exceptionally well, and can promote CD4+ T cell recognition and killing of biologically important host cells.</jats:p> | |
dc.format.extent | 1109-1119 | |
dc.format.medium | 8 | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Rockefeller University Press | |
dc.subject | Antibodies, Monoclonal | |
dc.subject | Antigen Presentation | |
dc.subject | CD4-Positive T-Lymphocytes | |
dc.subject | Cell Line | |
dc.subject | Endocytosis | |
dc.subject | Flow Cytometry | |
dc.subject | Histocompatibility Antigens Class II | |
dc.subject | Humans | |
dc.subject | Lymphocyte Subsets | |
dc.subject | Microscopy, Fluorescence | |
dc.subject | Neuroglia | |
dc.subject | T-Lymphocytes, Cytotoxic | |
dc.subject | Viral Envelope Proteins | |
dc.title | Endogenous human cytomegalovirus gB is presented efficiently by MHC class II molecules to CD4(+) CTL | |
dc.type | journal-article | |
dc.type | JOUR | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000232929500011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 8 | |
plymouth.volume | 202 | |
plymouth.publication-status | Published | |
plymouth.journal | Journal of Experimental Medicine | |
dc.identifier.doi | 10.1084/jem.20050162 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | United States | |
dc.identifier.eissn | 1540-9538 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1084/jem.20050162 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review |