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dc.contributor.authorMetwali, EMR
dc.contributor.authorGowayed, SMH
dc.contributor.authorAlmagrabi, OA
dc.contributor.authorMosleh, YY
dc.contributor.authorFuller, MP
dc.date.accessioned2013-11-17T09:38:11Z
dc.date.accessioned2013-11-17T09:39:02Z
dc.date.available2013-11-17T09:38:11Z
dc.date.available2013-11-17T09:39:02Z
dc.date.issued2013-11-13
dc.identifier.issn1459-0255
dc.identifier.issn1459-0263
dc.identifier.urihttp://hdl.handle.net/10026.1/2723
dc.description.abstract

Genetic analysis was studied in a 5 × 5 diallel cross following Hayman's diallel analysis approach to obtain additive and dominance components of genetic variance using a mature embryo culture protocol. Different concentrations of NaCl at 5 and 10 g L-1 were added to each medium. Callus fresh weight (g), shoot length (cm), root length (cm), proline (μg g-1), sodium (mg g-1 and potassium (mg g-1) content were determined. Also, screening for non-water-soluble protein polymorphism was carried out using leaves derived via callus culture and analysis using SDS-PAGE. Results on the genetic behaviour for previously traits, indicating the differences among the genotypes were significant (p ≤ 0.05) and highly significant (p ≤ 0.01). Wr/Vr graph in diallel analysis and average degree of dominance together with narrow-sense heritability values revealed additive gene effect for shoot length under control and saline treatments and callus fresh weight under control, while dominance gene action was detected for proline content under all treatments, K content under control and 5 g L-1, callus fresh weight under 5 g L-1 and root length under 10 g L-1 NaCl. The order of array along the regression line showed that G.123 followed by G.124 are more stable than other parents and could be recommended for use in the production of a hybrid breeding programme for salt tolerance. Based on SDS-PAGE, approximately 36 bands which arranged between 124 kDa to 12 kDa were exhibited in most of parents and their hybrids under different treatments. Out of these 36 bands, 8 bands were polymorphic and one of these bands at 34 kDa was absent only in sensitive parent (Beecher) under salt treatments, while another band at 42 kDa appeared in most of the parents and their hybrids under salt treatments.

dc.format.extent1386-1394
dc.languageEnglish
dc.language.isoEnglish
dc.publisherWFL publisher
dc.relation.replaceshttp://hdl.handle.net/10026.1/2722
dc.relation.replaces10026.1/2722
dc.titleEvaluation and selection of barley genotypes for salt stress resistance using tissue culture techniques and SDS-PAGE gel electrophoresis
dc.typejournal-article
dc.typeArticle
plymouth.issue3-4
plymouth.volume11 (3&4)
plymouth.publisher-urlhttp://www.world-food.net/
plymouth.publication-statusPublished
plymouth.journalJournal of Food, Agriculture and Environment
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeFinland
dc.identifier.eissn1459-0263
dc.rights.embargoperiodNot known
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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