Show simple item record

dc.contributor.authorJONES, JEFFREY WYNN
dc.contributor.otherPeninsula College of Medicine and Dentistryen_US
dc.date.accessioned2013-10-29T11:50:47Z
dc.date.accessioned2011-09-29T11:01:03Z
dc.date.available2013-10-29T11:50:47Z
dc.date.available2011-09-29T11:01:03Z
dc.date.issued1990
dc.identifierNOT AVAILABLEen_US
dc.identifier.urihttp://hdl.handle.net/10026.1/2440
dc.descriptionThis is a digitised version of a thesis that was deposited in the University Library. If you are the author and you have a query about this item please contact PEARL Admin (pearladmin@plymouth.ac.uk)
dc.descriptionMetadata merged with duplicate record (http://hdl.handle.net/10026.1/783) on 20.12.2016 by CS (TIS).
dc.description.abstract

A modified Ellman method using acetylthiocholine as substrata has been used to reliably measure erythrocyte AChE activity (CV=7%). Reliable immunological quantitation of this enzyme (CV=7%) was achieved using monoclonal antisera to AChE (AE-1 and AE-2) in a fluid phase homogeneous enzyme-linked antiglobulin test (ELAT). AChE activity and antigen levels were similar for both adult male and female samples. Cord samples at term measured 60% of the adult value and reached 100% within 5 months. An overall increase in AChE activity was observed as pregnancy progresses with patients 1 to 3 days post delivery showing significantly elevated levels of activity and antigen. Investigation of haematological disorders revealed cases of reduced levels of activity and antigen in a variety of conditions. An essay of plasma ChE activity using butyrylthiocholine as substrata has been developed using a microplate Ellman technique (CV=5%). An enzyme linked immunosorbent method (ELISA) using monoclonal antibodies MAb2-l and 2-4 (CV=5%) provided a sensitive and reliable means of quantitation together with the capability of handling a large number of samples. Normal Gaussian distribution curves were obtained for both activity and antigen levels for E1u E1u, E1uE1a and E1aE1a individuals. The approximate amount that each of the genes E1a , E1a and E1f contribute to the ChE activity and antigen levels is presented allowing the effective efficiency (EE) of the gene product to be calculated. Immunological measurements have aided in the segregation of the silent gene variants representing zero, very low, low and high levels of immunoreactive protein and the identification of a new gene, E1x. Family studies have confirmed the existence of this gene. Analysis of activity and immunological data suggest that the E1h and E1t gene could be identical. A significant decrease in ChE activity occurs during pregnancy with even lower levels presenting during the first 2 to 3 days post delivery. Immunological measurements indicate similar reductions in the amount of ChE protein during these periods.

en_US
dc.description.sponsorshipTorbay Hospitalen_US
dc.language.isoenen_US
dc.publisherUniversity of Plymouthen_US
dc.titleEnzyme Linked Immunological Studies of Cholinesterases in Human Blooden_US
dc.typeDoctorateen_US
plymouth.versionFull versionen_US


Files in this item

Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
@mire NV