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dc.contributor.authorBose, R
dc.contributor.authorIoannidis, K
dc.contributor.authorFoschi, Federico
dc.contributor.authorBakhsh, A
dc.contributor.authorKelly, RD
dc.contributor.authorDeb, S
dc.contributor.authorMannocci, F
dc.contributor.authorNiazi, SA
dc.date.accessioned2023-05-05T12:09:03Z
dc.date.available2023-05-05T12:09:03Z
dc.date.issued2020-09
dc.identifier.issn2077-0383
dc.identifier.issn2077-0383
dc.identifier.other2722
dc.identifier.urihttps://pearl.plymouth.ac.uk/handle/10026.1/20832
dc.description.abstract

Purpose: This study compared the antimicrobial efficacy of calcium silicate sealers (BioRoot RCS and Total Fill BC) and conventional sealers (AH Plus and Tubli-seal) against planktonic bacteria and a nutrient-stressed multispecies biofilm. Methods: Antimicrobial properties of freshly mixed sealers were investigated using the direct contact test (DCT) and a nutrient-stressed multispecies biofilm comprised of five endodontic strains. Antimicrobial activity was determined using quantitative viable counts and confocal laser scanning microscopy (CLSM) analysis with live/dead staining. The pH of the sealers was analysed over a period of 28 days in Hanks Balanced Salt Solution (HBSS). Analysis of variance (ANOVA) with Tukey tests and the Kruskal–Wallis test were used for data analysis with a significance of 5%. Results: All endodontic sealers exhibited significant antimicrobial activity against planktonic bacteria (p < 0.05). BioRoot RCS caused a significant reduction in viable counts of the biofilms compared to AH Plus and the control (p < 0.05), while no significant difference could be observed compared to TotalFill BC and Tubli-seal (p > 0.05). CLSM analysis showed that BioRoot RCS and TotalFill BC exhibited significant biofilm inhibition compared to Tubli-seal, AH Plus and the control (p < 0.05). BioRoot RCS presented with the highest microbial killing, followed by TotalFill BC and Tubli-seal. Alkalizing activity was seen from the onset by BioRoot RCS, TotalFill BC and AH Plus. After 28 days, BioRoot RCS demonstrated the highest pH in HBSS (pH > 12). Conclusions: Calcium silicate sealers exhibited effective antimicrobial properties. This was demonstrated by superior biofilm inhibition capacity and microbial killing, with strong alkalizing activity compared to epoxy-based and zinc oxide-eugenol-based sealers.

dc.format.extent2722-2722
dc.format.mediumElectronic
dc.languageen
dc.publisherMDPI AG
dc.subjectantimicrobial properties
dc.subjectbioceramic sealers
dc.subjectbiofilms
dc.subjectcalcium silicate sealers
dc.subjectconfocal laser scanning microscopy
dc.subjectdental materials
dc.subjectendodontic sealers
dc.titleAntimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000579914900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue9
plymouth.volume9
plymouth.publication-statusPublished online
plymouth.journalJournal of Clinical Medicine
dc.identifier.doi10.3390/jcm9092722
plymouth.organisational-group|Plymouth
plymouth.organisational-group|Plymouth|Faculty of Health
plymouth.organisational-group|Plymouth|Users by role
plymouth.organisational-group|Plymouth|Users by role|Academics
plymouth.organisational-group|Plymouth|Faculty of Health|Peninsula Dental School
dc.publisher.placeSwitzerland
dcterms.dateAccepted2020-08-21
dc.date.updated2023-05-05T12:08:45Z
dc.rights.embargodate2023-8-5
dc.identifier.eissn2077-0383
dc.rights.embargoperiodforever
rioxxterms.versionofrecord10.3390/jcm9092722


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