Antimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm
dc.contributor.author | Bose, R | |
dc.contributor.author | Ioannidis, K | |
dc.contributor.author | Foschi, Federico | |
dc.contributor.author | Bakhsh, A | |
dc.contributor.author | Kelly, RD | |
dc.contributor.author | Deb, S | |
dc.contributor.author | Mannocci, F | |
dc.contributor.author | Niazi, SA | |
dc.date.accessioned | 2023-05-05T12:09:03Z | |
dc.date.available | 2023-05-05T12:09:03Z | |
dc.date.issued | 2020-09 | |
dc.identifier.issn | 2077-0383 | |
dc.identifier.issn | 2077-0383 | |
dc.identifier.other | 2722 | |
dc.identifier.uri | https://pearl.plymouth.ac.uk/handle/10026.1/20832 | |
dc.description.abstract |
Purpose: This study compared the antimicrobial efficacy of calcium silicate sealers (BioRoot RCS and Total Fill BC) and conventional sealers (AH Plus and Tubli-seal) against planktonic bacteria and a nutrient-stressed multispecies biofilm. Methods: Antimicrobial properties of freshly mixed sealers were investigated using the direct contact test (DCT) and a nutrient-stressed multispecies biofilm comprised of five endodontic strains. Antimicrobial activity was determined using quantitative viable counts and confocal laser scanning microscopy (CLSM) analysis with live/dead staining. The pH of the sealers was analysed over a period of 28 days in Hanks Balanced Salt Solution (HBSS). Analysis of variance (ANOVA) with Tukey tests and the Kruskal–Wallis test were used for data analysis with a significance of 5%. Results: All endodontic sealers exhibited significant antimicrobial activity against planktonic bacteria (p < 0.05). BioRoot RCS caused a significant reduction in viable counts of the biofilms compared to AH Plus and the control (p < 0.05), while no significant difference could be observed compared to TotalFill BC and Tubli-seal (p > 0.05). CLSM analysis showed that BioRoot RCS and TotalFill BC exhibited significant biofilm inhibition compared to Tubli-seal, AH Plus and the control (p < 0.05). BioRoot RCS presented with the highest microbial killing, followed by TotalFill BC and Tubli-seal. Alkalizing activity was seen from the onset by BioRoot RCS, TotalFill BC and AH Plus. After 28 days, BioRoot RCS demonstrated the highest pH in HBSS (pH > 12). Conclusions: Calcium silicate sealers exhibited effective antimicrobial properties. This was demonstrated by superior biofilm inhibition capacity and microbial killing, with strong alkalizing activity compared to epoxy-based and zinc oxide-eugenol-based sealers. | |
dc.format.extent | 2722-2722 | |
dc.format.medium | Electronic | |
dc.language | en | |
dc.publisher | MDPI AG | |
dc.subject | antimicrobial properties | |
dc.subject | bioceramic sealers | |
dc.subject | biofilms | |
dc.subject | calcium silicate sealers | |
dc.subject | confocal laser scanning microscopy | |
dc.subject | dental materials | |
dc.subject | endodontic sealers | |
dc.title | Antimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm | |
dc.type | journal-article | |
dc.type | Journal Article | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000579914900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 9 | |
plymouth.volume | 9 | |
plymouth.publication-status | Published online | |
plymouth.journal | Journal of Clinical Medicine | |
dc.identifier.doi | 10.3390/jcm9092722 | |
plymouth.organisational-group | |Plymouth | |
plymouth.organisational-group | |Plymouth|Faculty of Health | |
plymouth.organisational-group | |Plymouth|Users by role | |
plymouth.organisational-group | |Plymouth|Users by role|Academics | |
plymouth.organisational-group | |Plymouth|Faculty of Health|Peninsula Dental School | |
dc.publisher.place | Switzerland | |
dcterms.dateAccepted | 2020-08-21 | |
dc.date.updated | 2023-05-05T12:08:45Z | |
dc.rights.embargodate | 2023-8-5 | |
dc.identifier.eissn | 2077-0383 | |
dc.rights.embargoperiod | forever | |
rioxxterms.versionofrecord | 10.3390/jcm9092722 |