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dc.contributor.authorHadi, HA
dc.contributor.authorSmerdon, G
dc.contributor.authorW Fox, S
dc.date.accessioned2023-02-15T15:18:54Z
dc.date.available2023-02-15T15:18:54Z
dc.date.issued2015-03-04
dc.identifier.issn1745-3674
dc.identifier.issn1745-3682
dc.identifier.urihttp://hdl.handle.net/10026.1/20384
dc.description.abstract

BACKGROUND AND PURPOSE: Hypoxia, necrosis, and bone loss are hallmarks of many skeletal diseases. Hyperbaric oxygen therapy (HBO) is often used as an adjunctive therapy in these cases. However the in vivo effect of HBO on osteoclast formation has not been fully established. We therefore carried out a longitudinal study to examine the effect of HBO on osteoclast formation and bone resorptive capacity in patients who were referred to the Plymouth Hyperbaric Medical Centre. METHODS: Osteoclast precursors were isolated from peripheral blood prior to and following 10 and 25 daily hyperbaric treatments (100% O2 at 2.4 atmospheres absolute ATA for 90 min) to determine osteoclast formation and resorptive capacity. The expression of key regulators of osteoclast differentiation RANK, Dc-STAMP, and NFATc1 was also assessed by quantitative real-time PCR. RESULTS: HBO reduced the ability of precursors to form osteoclasts and reduced bone resorption in a treatment-dependent manner. The initial suppressive effect of HBO was more pronounced on mononuclear osteoclast formation than on multinuclear osteoclast formation, and this was accompanied by reduction in the expression of key regulators of osteoclast formation, RANK and Dc-STAMP. INTERPRETATION: This study shows for the first time that in vivo, HBO suppresses the ability of monocytic precursors to form resorptive osteoclasts.

dc.format.extent264-269
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherMedical Journals Sweden AB
dc.subjectAdaptor Proteins, Signal Transducing
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectBone Resorption
dc.subjectCell Differentiation
dc.subjectCells, Cultured
dc.subjectChronic Disease
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectHyperbaric Oxygenation
dc.subjectLongitudinal Studies
dc.subjectMale
dc.subjectMembrane Proteins
dc.subjectMiddle Aged
dc.subjectMonocytes
dc.subjectNFATC Transcription Factors
dc.subjectOsteoclasts
dc.subjectRNA, Messenger
dc.subjectRadiation Injuries
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectReceptor Activator of Nuclear Factor-kappa B
dc.subjectWounds and Injuries
dc.titleOsteoclastic resorptive capacity is suppressed in patients receiving hyperbaric oxygen therapy
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/25238438
plymouth.issue2
plymouth.volume86
plymouth.publisher-urlhttp://dx.doi.org/10.3109/17453674.2014.964621
plymouth.publication-statusPublished
plymouth.journalActa Orthopaedica
dc.identifier.doi10.3109/17453674.2014.964621
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR)
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeSweden
dc.identifier.eissn1745-3682
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.3109/17453674.2014.964621
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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