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dc.contributor.authorHicks, JM
dc.contributor.authorSilman, NJ
dc.contributor.authorJackson, SK
dc.contributor.authorAylott, JW
dc.contributor.authorRawson, FJ
dc.date.accessioned2022-11-16T17:01:34Z
dc.date.issued2020-10
dc.identifier.issn1567-5394
dc.identifier.issn1878-562X
dc.identifier.other107547
dc.identifier.urihttp://hdl.handle.net/10026.1/19982
dc.description.abstract

Hydrogen peroxide is a key component of the innate immune response, regulating how a cell responds to a bacterial threat; however, being transient in nature makes it extremely difficult to detect. We show the development of an improved biosensor capable of the rapid detection of the hydrogen peroxide produced intracellularly in response to both smooth and rough lipopolysaccharides (LPS) structures. The arising signal and mass transport behaviour to the electrodes were characterised. This response was detected utilising a single walled carbon nanotube-based sensor that has been functionalised with an osmium complex for specificity and detecting the change in intracellular concentrations of hydrogen peroxide through chronoamperometry. This was conducted within murine macrophage (RAW264.7) cells and using ultra-pure LPS extracted from two different serotypes of bacteria (0111:B4 and Re495). This allowed the comparison of the immune response when infected with different structures of LPS. We demonstrate that the hydrogen peroxide signal can be electrochemically detected within 3 seconds post injection. Combining the nature of the mass transport of hydrogen peroxide and concentration characteristics, a bacterial 'fingerprint' was identified. The impact of this work will be demonstrated in allowing us to develop a rapid diagnostic for bacterial detection.

dc.format.extent107547-107547
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherElsevier BV
dc.subjectLPS
dc.subjectBiosensor
dc.subjectHydrogen peroxide
dc.subjectCarbon nanotubes
dc.subjectDiffusion
dc.titleMass transport of lipopolysaccharide induced H2O2 detected by an intracellular carbon nanoelectrode sensor
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000579730600024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.volume135
plymouth.publication-statusPublished
plymouth.journalBioelectrochemistry
dc.identifier.doi10.1016/j.bioelechem.2020.107547
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
plymouth.organisational-group/Plymouth/Users by role/Researchers in ResearchFish submission
dc.publisher.placeNetherlands
dcterms.dateAccepted2020-04-30
dc.rights.embargodate9999-12-31
dc.identifier.eissn1878-562X
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1016/j.bioelechem.2020.107547
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-10
rioxxterms.typeJournal Article/Review


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