Show simple item record

dc.contributor.authorMetwali, EMR
dc.contributor.authorKadasa, NMS
dc.contributor.authorSoliman, HIA
dc.contributor.authorAlmaghrabi, OA
dc.contributor.authorFuller, MP::0000-0003-3461-9728

To overcome the genetic deterioration and the extinction of date palm genotypes and species as result of environmental challenges, it has become necessary to develop techniques that allow the remaining genetic resources to be persevered under in-vitro conditions for long period without a substantial decline in the vitality, genetic stability and low survival. The development of an effective cryopreservation method for date palm (cv. Magdoul) via an encapsulation-dehydration method for long term conservation was researched in this study. Embryogenic callus, obtained from shoot tip culture, was used as explants and exposed to different concentration of sucrose (0.0, 0.5, 0.75, 1.0 and 1.5 M) combined with dehydration time (0–10 days) and different drying time (1–10 h). It was found that using sucrose at 0.75 M was more effective compared with using 1.0 and 1.5 M. Among the drying time tested, 4 h gave the best result for survival. The interaction treatment between sucrose, dehydration and moisture content (MC) was studies. After different periods of time in liquid nitrogen, the greatest values of survival (74.4%) and regrowth (71.25%) after 6 weeks of storage were obtained when 0.75 M sucrose for three days followed by 4 h dehydration period with a 39.50% MC was applied. The present results indicated that encapsulation-dehydration can be applied as a simple and effective protocol to a diverse range of cv. Magdoul genetic resources using embryogenic calli. © 2020 Friends Science Publishers

dc.publisherSmith and Franklin Academic Publishing
dc.titleCryopreservation of Embryogenic Callus of Date Palm (Phoenix dactylifera) cv. Magdoul through Encapsulation-Dehydration Technology
dc.typeJournal Article
plymouth.journalInternational Journal of Agriculture and Biology
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.rights.embargoperiodNot known
rioxxterms.typeJournal Article/Review

Files in this item


This item appears in the following Collection(s)

Show simple item record

All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
Atmire NV