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dc.contributor.authorAljafer, Naofel
dc.contributor.authorDickinson, M
dc.date.accessioned2022-11-07T11:13:56Z
dc.date.available2022-11-07T11:13:56Z
dc.date.issued2021-11
dc.identifier.issn1125-4653
dc.identifier.issn2239-7264
dc.identifier.urihttp://hdl.handle.net/10026.1/19854
dc.description.abstract

A new set of loop-mediated isothermal amplification assays was developed for detection of phytoplasmas associated with plant diseases and optimised for rapid laboratory detection. This set of assays was designed based on the leucyl tRNA synthetase gene expressed in most of the phytoplasmas and showing a good level of sequence divergence among strains from different 16Sr groups. This makes it a good target for developing discrete assays that can detect specific phytoplasma 16Sr groups. In this study, unique sets of LAMP assays were designed and validated for seven phytoplasma ribosomal groups (16SrI, 16SrII, 16SrIII, 16SrV, 16SrVI, 16SrX, and 16SrXII). These assays are specific, rapid and as sensitive as the nested PCR assays and the results could be obtained in less than 30 minutes in real-time LAMP assays.

dc.format.extent1315-1321
dc.languageen
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.subject16S-23S ITS region
dc.subjectLeucyl tRNA synthetase gene
dc.subjectRapid detection
dc.titleEvaluating LAMP assays for detection of phytoplasmas classified in different ribosomal groups
dc.typejournal-article
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000674567900002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue4
plymouth.volume103
plymouth.publication-statusPublished
plymouth.journalJournal of Plant Pathology
dc.identifier.doi10.1007/s42161-021-00906-1
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dcterms.dateAccepted2021-07-05
dc.rights.embargodate2022-11-23
dc.identifier.eissn2239-7264
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1007/s42161-021-00906-1
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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