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dc.contributor.authorWaterfield, S
dc.contributor.authorAhmed, H
dc.contributor.authorJones, IA
dc.contributor.authorBurky, R
dc.contributor.authorJoshi, LT
dc.date.accessioned2022-04-25T09:37:51Z
dc.date.issued2022-06-08
dc.identifier.issn0022-2615
dc.identifier.issn1473-5644
dc.identifier.otherARTN 001550
dc.identifier.urihttp://hdl.handle.net/10026.1/19058
dc.description.abstract

<jats:p> <jats:bold>Background.</jats:bold> <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">Clostridioides difficile</jats:ext-link> </jats:named-content> </jats:italic> is a spore-forming pathogen responsible for antibiotic-associated diarrhoea. In the USA high incidence of <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> infection (CDI) in clinical environments has led to interest in <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> spore transmission.</jats:p> <jats:p> <jats:bold>Hypothesis.</jats:bold> Single use hospital surgical gown ties act as a reservoir for <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> spores.</jats:p> <jats:p> <jats:bold>Aim.</jats:bold> This study sought to examine whether single-use hospital surgical gown ties used in surgery, from an acute healthcare facility, harboured <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> spores.</jats:p> <jats:p> <jats:bold>Methodology.</jats:bold> Used surgical gowns ties worn by clinicians in the healthcare facility were examined for <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> spore presence via spread plate and anaerobic culture. The colonies isolated from each gown tie were subcultured on <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> selective agar for phenotypic confirmation. Presumptive <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> colonies were examined using <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> Quik Check Complete, 16–23S PCR Ribotyping and MALDI-TOF analysis.</jats:p> <jats:p> <jats:bold>Results.</jats:bold> In total 17 suspected <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> colonies were isolated from 15 gown ties via culture. <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> Quik Check Complete found two isolates as possible <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic>. MALDI-TOF and PCR Ribotyping confirmed one isolate as <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> PCR ribotype 027 associated with clinical outbreaks.</jats:p> <jats:p> <jats:bold>Discussion.</jats:bold> Our study revealed the presence of hypervirulent <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28959" xlink:type="simple">C. difficile</jats:ext-link> </jats:named-content> </jats:italic> ribotype 027 spores on single-use gown ties. This highlights the potential of gown ties as a vector of spore transmission across clinical environments, especially when gowns are not worn appropriately.</jats:p> <jats:p> <jats:bold>Conclusions.</jats:bold> Appropriate compliance to infection control procedures by healthcare workers is essential to prevent spore dissemination across clinical facilities and reduce CDI rates.</jats:p>

dc.format.mediumPrint
dc.languageen
dc.language.isoen
dc.publisherMicrobiology Society
dc.subjectClostridioides difficile
dc.subjecttransmission
dc.subjectspores
dc.subjectgowns
dc.subjectflooring
dc.subjectinfection control
dc.titleIsolation of Clostridioides difficile PCR Ribotype 027 from single-use hospital gown ties
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35675100
plymouth.issue6
plymouth.volume71
plymouth.publisher-urlhttp://dx.doi.org/10.1099/jmm.0.001550
plymouth.publication-statusPublished
plymouth.journalJournal of Medical Microbiology
dc.identifier.doi10.1099/jmm.0.001550
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeEngland
dcterms.dateAccepted2022-04-20
dc.rights.embargodate2022-6-14
dc.identifier.eissn1473-5644
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1099/jmm.0.001550
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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