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dc.contributor.authorSafarzadeh, Mina
dc.contributor.authorPan, Genhua
dc.date.accessioned2022-02-08T16:57:32Z
dc.date.available2022-02-08T16:57:32Z
dc.date.issued2022-02-05
dc.identifier.issn2079-6374
dc.identifier.issn2079-6374
dc.identifier.otherARTN 98
dc.identifier.urihttp://hdl.handle.net/10026.1/18720
dc.description.abstract

<jats:p>The ability to detect double-stranded DNA (dsDNA) as a biomarker without denaturing it to single-stranded DNA (ss-DNA) continues to be a major challenge. In this work, we report a sandwich biosensor for the detection of the ds-methylated MGMT gene, a potential biomarker for brain tumors and breast cancer. The purpose of this biosensor is to achieve simultaneous recognition of the gene sequence, as well as the presence of methylation. The biosensor is based on reduced graphene oxide (rGO) electrodes decorated with gold nanoparticles (AuNPs) and uses Peptide Nucleic Acid (PNA) that binds to the ds-MGMT gene. The reduction of GO was performed in two ways: electrochemically (ErGO) and thermally (TrGO). XPS and Raman spectroscopy, as well as voltammetry techniques, showed that the ErGO was more efficiently reduced, had a higher C/O ratio, showed a smaller crystallite size of the sp2 lattice, and was more stable during measurement. It was also revealed that the electro-deposition of the AuNPs was more successful on the ErGO surface due to the higher At% of Au on the ErGO electrode. Therefore, the ErGO/AuNPs electrode was used to develop biosensors to detect the ds-MGMT gene. PNA, which acts as a bio-recognition element, was used to form a self-assembled monolayer (SAM) on the ErGO/AuNPs surface via the amine-AuNPs interaction, recognizing the ds-MGMT gene sequence by its invasion of the double-stranded DNA and the formation of a triple helix. The methylation was then detected using biotinylated-anti-5mC, which was then measured using the amperometric technique. The selectivity study showed that the proposed biosensor was able to distinguish between blank, non-methylated, non-complementary, and target dsDNA spiked in mouse plasma. The LOD was calculated to be 0.86 pM with a wide linear range of 1 pM to 50 µM. To the best of our knowledge, this is the first report on using PNA to detect ds-methylated DNA. This sandwich design can be modified to detect other methylated genes, making it a promising platform to detect ds-methylated biomarkers.</jats:p>

dc.format.extent98-98
dc.format.mediumElectronic
dc.languageen
dc.language.isoen
dc.publisherMDPI AG
dc.rightsAttribution-NonCommercial 4.0 International
dc.rightsAttribution-NonCommercial 4.0 International
dc.rightsAttribution-NonCommercial 4.0 International
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectelectrochemical reduction of graphene oxide
dc.subjectrGO
dc.subjectPNA
dc.subjectdetection of double-stranded DNA
dc.subjectMGMT
dc.titleDetection of a Double-Stranded MGMT Gene Using Electrochemically Reduced Graphene Oxide (ErGO) Electrodes Decorated with AuNPs and Peptide Nucleic Acids (PNA)
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000770862900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue2
plymouth.volume12
plymouth.publication-statusPublished online
plymouth.journalBiosensors
dc.identifier.doi10.3390/bios12020098
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Engineering, Computing and Mathematics
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA12 Engineering
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeSwitzerland
dcterms.dateAccepted2022-02-01
dc.rights.embargodate2022-2-10
dc.identifier.eissn2079-6374
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.3390/bios12020098
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc/4.0/
rioxxterms.licenseref.startdate2022-02-05
rioxxterms.typeJournal Article/Review
plymouth.funderAn Integrated Platform for Developing Brain Cancer Diagnostic Techniques::European Commission Directorate-General for Research and Innovation


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