Show simple item record

dc.contributor.authorHall, JPJ
dc.contributor.authorWright, RCT
dc.contributor.authorHarrison, E
dc.contributor.authorMuddiman, Katie
dc.contributor.authorWood, AJ
dc.contributor.authorPaterson, S
dc.contributor.authorBrockhurst, MA
dc.date.accessioned2022-02-04T10:05:40Z
dc.date.issued2021-10-13
dc.identifier.issn1544-9173
dc.identifier.issn1545-7885
dc.identifier.othere3001225
dc.identifier.urihttp://hdl.handle.net/10026.1/18643
dc.description.abstract

<jats:p>Plasmids play an important role in bacterial genome evolution by transferring genes between lineages. Fitness costs associated with plasmid carriage are expected to be a barrier to gene exchange, but the causes of plasmid fitness costs are poorly understood. Single compensatory mutations are often sufficient to completely ameliorate plasmid fitness costs, suggesting that such costs are caused by specific genetic conflicts rather than generic properties of plasmids, such as their size, metabolic burden, or gene expression level. By combining the results of experimental evolution with genetics and transcriptomics, we show here that fitness costs of 2 divergent large plasmids in <jats:italic>Pseudomonas fluorescens</jats:italic> are caused by inducing maladaptive expression of a chromosomal tailocin toxin operon. Mutations in single genes unrelated to the toxin operon, and located on either the chromosome or the plasmid, ameliorated the disruption associated with plasmid carriage. We identify one of these compensatory loci, the chromosomal gene <jats:italic>PFLU4242</jats:italic>, as the key mediator of the fitness costs of both plasmids, with the other compensatory loci either reducing expression of this gene or mitigating its deleterious effects by up-regulating a putative plasmid-borne ParAB operon. The chromosomal mobile genetic element Tn6291, which uses plasmids for transmission, remained up-regulated even in compensated strains, suggesting that mobile genetic elements communicate through pathways independent of general physiological disruption. Plasmid fitness costs caused by specific genetic conflicts are unlikely to act as a long-term barrier to horizontal gene transfer (HGT) due to their propensity for amelioration by single compensatory mutations, helping to explain why plasmids are so common in bacterial genomes.</jats:p>

dc.format.extente3001225-e3001225
dc.format.mediumElectronic-eCollection
dc.languageen
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.subjectChromosomes, Bacterial
dc.subjectConjugation, Genetic
dc.subjectEvolution, Molecular
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenetic Fitness
dc.subjectModels, Biological
dc.subjectMutation
dc.subjectPlasmids
dc.subjectPseudomonas fluorescens
dc.subjectTranscription, Genetic
dc.subjectUp-Regulation
dc.titlePlasmid fitness costs are caused by specific genetic conflicts enabling resolution by compensatory mutation
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000708845500002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue10
plymouth.volume19
plymouth.publication-statusPublished online
plymouth.journalPLOS Biology
dc.identifier.doi10.1371/journal.pbio.3001225
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Users by role
dc.publisher.placeUnited States
dcterms.dateAccepted2021-09-20
dc.rights.embargodate2023-7-4
dc.identifier.eissn1545-7885
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1371/journal.pbio.3001225
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-10
rioxxterms.typeJournal Article/Review


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
Atmire NV