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dc.contributor.authorPlayford, Raymond
dc.contributor.authorCattell, M
dc.contributor.authorMarchbank, T
dc.date.accessioned2021-10-19T12:35:09Z
dc.date.issued2020-06-17
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.othere0234719
dc.identifier.urihttp://hdl.handle.net/10026.1/18117
dc.description.abstract

BACKGROUND: Colostrum, the milk produced during first few days after birth, is rich in immunoglobulins, antimicrobial peptides & growth factors. Multiple clinical trials using bovine colostrum are ongoing but with no assessment of test product bioactivity. OBJECTIVES: To examine variability of bioactivity between 20 commercial colostrum products, contribution of TGFβ and EGFR in mediating effects, heat sensitivity of bioactivity and changes in bioactivity of colostrum milkings in the days following calving. DESIGN: In vitro bioactivity used AGS, RIE-1 and Caco-2 cell proliferation (Alamar blue) and migration (wounded monolayers) assays. Changes in colostrum bioactivity determined following addition of TGFβ-neutralising antibody, EGFR blocker (Typhostin) and after heating (40-60°C, 60 min). In vivo bioassay assessed ability of colostrum gavage (2ml, 7mg/ml) to reduce gastric damage (NSAID + restraint) in rats. Milkings from 6 cows, days 0-3 post calving were assessed for bioactivity and growth factor concentrations. RESULT: Six-fold differences in pro-proliferative and migratory activity were seen comparing commercial products. Comparison of most- and least-active samples from in vitro studies showed two- to three-fold differences in ability to reduce gastric injury (86% reduction using most-active vs 48% using least-active, p<0.01). Tyrphostin reduced pro-migratory and proliferative activity by 23% and 55%. TGFβ neutralisation reduced migratory activity by 83% but did not affect proliferation Heating colostrum powder to 50°C did not affect immunoactivity of haptoglobin, EGF, TGFβ, IgG, IGF-1 or betacellulin but decreased bioactivity by >40%. Milking studies showed high bioactivity during first and second milkings on day 0 but 77% reduction by day 3. Changes in total protein, haptoglobin, EGF, TGFβ, IgG and IGF-1 paralleled falls in bioactivity. CONCLUSION: Commercial colostrum products possess widely different bioactivity. Variation in heat exposure and/or proportion of day 0 colostrum content may contribute to this. Assessment of colostrum bioactivity has advantages to growth factor quantitation for quality control.

dc.format.extente0234719-e0234719
dc.format.mediumElectronic-eCollection
dc.languageen
dc.language.isoen
dc.publisherPublic Library of Science
dc.subjectAged
dc.subjectAnimals
dc.subjectCaco-2 Cells
dc.subjectCattle
dc.subjectCell Movement
dc.subjectCell Proliferation
dc.subjectClinical Trials as Topic
dc.subjectColostrum
dc.subjectErbB Receptors
dc.subjectFemale
dc.subjectHot Temperature
dc.subjectHumans
dc.subjectMale
dc.subjectPregnancy
dc.subjectTransforming Growth Factor beta
dc.titleMarked variability in bioactivity between commercially available bovine colostrum for human use; implications for clinical trials
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/32555629
plymouth.issue6
plymouth.volume15
plymouth.publication-statusPublished online
plymouth.journalPLoS One
dc.identifier.doi10.1371/journal.pone.0234719
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
dc.publisher.placeUnited States
dcterms.dateAccepted2020-06-02
dc.rights.embargodate2021-10-20
dc.identifier.eissn1932-6203
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1371/journal.pone.0234719
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-06-17
rioxxterms.typeJournal Article/Review


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