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dc.contributor.authorTrotman-Lucas, M
dc.contributor.authorKelly, ME
dc.contributor.authorJanus, J
dc.contributor.authorfern, robert
dc.contributor.authorGibson, CL
dc.date.accessioned2021-09-17T13:08:17Z
dc.date.available2021-09-17T13:08:17Z
dc.date.issued2017-07
dc.identifier.issn1754-8403
dc.identifier.issn1754-8411
dc.identifier.urihttp://hdl.handle.net/10026.1/17832
dc.description.abstract

<jats:p>Animal models are critical for understanding the pathology of stroke and investigating potential treatments. However, in vivo stroke models are associated, particularly in mice, with high variability in lesion volume. We investigated whether a surgical refinement where reperfusion is not reliant on the Circle of Willis reduced outcome variability. Mice underwent 60 minutes of transient middle cerebral artery occlusion avoiding ligation of the external carotid artery. During reperfusion the common carotid artery was either ligated (standard approach), or it was repaired to allow re-establishment of blood flow through the common carotid artery. All mice underwent MRI scanning for assessment of infarct volume, apparent diffusion coefficient and fractional anisotropy along with terminal assessment of infarct volume by TTC staining. Repairing the common carotid artery following middle cerebral artery occlusion enhanced reperfusion (P&amp;lt;0.01) and reduced the variability seen in both total (histological analysis, P =0.008; T2-weighted MRI, P=0.015) and core (diffusion tensor imaging MRI scanning, P=0.043) lesion volume. Avoiding external carotid artery ligation may improve animal wellbeing, through reduced weight loss, while using an alternative surgical approach which enabled reperfusion through the common carotid artery decreased the variability in lesion volume seen within groups.</jats:p>

dc.format.extent931-938
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherCompany of Biologists
dc.subjectAnimal models
dc.subjectBrain ischemia
dc.subjectMRI
dc.subjectTransient MCAO
dc.subjectCarotid artery ligation
dc.titleAn alternative surgical approach reduces variability following filament induction of experimental stroke in mice
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000404963600010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue7
plymouth.volume10
plymouth.publication-statusPublished
plymouth.journalDisease Models and Mechanisms
dc.identifier.doi10.1242/dmm.029108
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Admin Group - REF
plymouth.organisational-group/Plymouth/Admin Group - REF/REF Admin Group - FoH
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeEngland
dcterms.dateAccepted2017-05-24
dc.rights.embargodate2021-9-18
dc.identifier.eissn1754-8411
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1242/dmm.029108
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2017-07
rioxxterms.typeJournal Article/Review


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