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dc.contributor.authorRobinson, CV
dc.contributor.authorBishop, Alistair
dc.date.accessioned2021-09-17T12:25:37Z
dc.date.issued2019-06
dc.identifier.issn1364-5072
dc.identifier.issn1365-2672
dc.identifier.urihttp://hdl.handle.net/10026.1/17818
dc.description.abstract

Aims To develop a gel formulation to trigger a visual signal for rapid disclosure of the location and extent of surface contamination with viable Bacillus anthracis spores.

Methods and Results Methylumbelliferyl‐α‐D‐glucopyranoside was combined with hyaluronic acid to produce a gel that could be applied to a surface as a coating. It remained hydrated for a sufficient time for α‐glucosidase activity present in intact B. anthracis spores to cleave the substrate and release the fluorescent product, methylumbelliferone. The presence of B. anthracis spores could be disclosed at 5 × 104 CFU per reaction test well (0·32 cm2) both visually and using fluorescence detection equipment.

Conclusions The disclosure gel provides a rapid, visual response to the presence of B. anthracis spores on a surface.

Significance and Impact of the Study The disclosure gel demonstrates the first steps towards the development of a formulation that can provide nonspecialist users with a visual alert to the presence of B. anthracis spores on a surface. It is envisioned that such a formulation would be beneficial in scenarios where exposure to spore release is a risk, and could be used in the initial assessment of equipment to aid prioritization and localized execution of a decontamination strategy.

dc.format.extent1700-1707
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherWiley
dc.subjectBacillus
dc.subjectdetection
dc.subjectenzymes
dc.subjectmicrobial contamination
dc.subjectspores
dc.titleA disclosure gel for visual detection of liveBacillus anthracisspores
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000467570600006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue6
plymouth.volume126
plymouth.publication-statusPublished
plymouth.journalJournal of Applied Microbiology
dc.identifier.doi10.1111/jam.14226
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
dc.publisher.placeEngland
dcterms.dateAccepted2019-02-14
dc.rights.embargodate2021-9-18
dc.identifier.eissn1365-2672
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1111/jam.14226
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-06
rioxxterms.typeJournal Article/Review


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