Show simple item record

dc.contributor.authorMarchetti, Ren
dc.contributor.authorDillon, MJen
dc.contributor.authorBurtnick, MNen
dc.contributor.authorHubbard, MAen
dc.contributor.authorKenfack, MTen
dc.contributor.authorBlériot, Yen
dc.contributor.authorGauthier, Cen
dc.contributor.authorBrett, PJen
dc.contributor.authorAuCoin, DPen
dc.contributor.authorLanzetta, Ren
dc.contributor.authorSilipo, Aen
dc.contributor.authorMolinaro, Aen

Burkholderia pseudomallei is the bacterium responsible for melioidosis, an infectious disease with high mortality rates. Since melioidosis is a significant public health concern in endemic regions and the organism is currently classified as a potential biothreat agent, the development of effective vaccines and rapid diagnostics is a priority. The capsular polysaccharide (CPS) expressed by B. pseudomallei is a highly conserved virulence factor and a protective antigen. Because of this, CPS is considered an attractive antigen for use in the development of both vaccines and diagnostics. In the present study, we describe the interactions of CPS with the murine monoclonal antibody (mAb) 4C4 using a multidisciplinary approach including organic synthesis, molecular biology techniques, surface plasmon resonance, and nuclear magnetic spectroscopy. Using these methods, we determined the mode of binding between mAb 4C4 and native CPS or ad hoc synthesized capsular polysaccharide fragments. Interestingly, we demonstrated that the O-acetyl moiety of CPS is essential for the interaction of the CPS epitope with mAb 4C4. Collectively, our results provide important insights into the structural features of B. pseudomallei CPS that enable antibody recognition that may help the rational design of CPS-based vaccine candidates. In addition, our findings confirm that the mAb 4C4 is suitable for use in an antibody-based detection assay for diagnosis of B. pseudomallei infections.

dc.format.extent2295 - 2302en
dc.subjectAntibodies, Monoclonalen
dc.subjectBacterial Capsulesen
dc.subjectBacterial Vaccinesen
dc.subjectBiological Warfare Agentsen
dc.subjectBurkholderia pseudomalleien
dc.subjectProtein Bindingen
dc.titleBurkholderia pseudomallei Capsular Polysaccharide Recognition by a Monoclonal Antibody Reveals Key Details toward a Biodefense Vaccine and Diagnostics against Melioidosis.en
dc.typeJournal Article
plymouth.journalACS Chem Biolen
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited Statesen
dc.rights.embargoperiodNot knownen
rioxxterms.typeJournal Article/Reviewen

Files in this item


This item appears in the following Collection(s)

Show simple item record

All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
@mire NV