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dc.contributor.authorMarchetti, Ren
dc.contributor.authorDillon, MJen
dc.contributor.authorBurtnick, MNen
dc.contributor.authorHubbard, MAen
dc.contributor.authorKenfack, MTen
dc.contributor.authorBlériot, Yen
dc.contributor.authorGauthier, Cen
dc.contributor.authorBrett, PJen
dc.contributor.authorAuCoin, DPen
dc.contributor.authorLanzetta, Ren
dc.contributor.authorSilipo, Aen
dc.contributor.authorMolinaro, Aen
dc.date.accessioned2021-09-16T14:37:16Z
dc.date.available2021-09-16T14:37:16Z
dc.date.issued2015-10-16en
dc.identifier.urihttp://hdl.handle.net/10026.1/17808
dc.description.abstract

Burkholderia pseudomallei is the bacterium responsible for melioidosis, an infectious disease with high mortality rates. Since melioidosis is a significant public health concern in endemic regions and the organism is currently classified as a potential biothreat agent, the development of effective vaccines and rapid diagnostics is a priority. The capsular polysaccharide (CPS) expressed by B. pseudomallei is a highly conserved virulence factor and a protective antigen. Because of this, CPS is considered an attractive antigen for use in the development of both vaccines and diagnostics. In the present study, we describe the interactions of CPS with the murine monoclonal antibody (mAb) 4C4 using a multidisciplinary approach including organic synthesis, molecular biology techniques, surface plasmon resonance, and nuclear magnetic spectroscopy. Using these methods, we determined the mode of binding between mAb 4C4 and native CPS or ad hoc synthesized capsular polysaccharide fragments. Interestingly, we demonstrated that the O-acetyl moiety of CPS is essential for the interaction of the CPS epitope with mAb 4C4. Collectively, our results provide important insights into the structural features of B. pseudomallei CPS that enable antibody recognition that may help the rational design of CPS-based vaccine candidates. In addition, our findings confirm that the mAb 4C4 is suitable for use in an antibody-based detection assay for diagnosis of B. pseudomallei infections.

en
dc.format.extent2295 - 2302en
dc.languageengen
dc.language.isoengen
dc.subjectAntibodies, Monoclonalen
dc.subjectBacterial Capsulesen
dc.subjectBacterial Vaccinesen
dc.subjectBiological Warfare Agentsen
dc.subjectBurkholderia pseudomalleien
dc.subjectHumansen
dc.subjectImmunoblottingen
dc.subjectMelioidosisen
dc.subjectProtein Bindingen
dc.titleBurkholderia pseudomallei Capsular Polysaccharide Recognition by a Monoclonal Antibody Reveals Key Details toward a Biodefense Vaccine and Diagnostics against Melioidosis.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/26198038en
plymouth.issue10en
plymouth.volume10en
plymouth.publication-statusPublisheden
plymouth.journalACS Chem Biolen
dc.identifier.doi10.1021/acschembio.5b00502en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited Statesen
dc.identifier.eissn1554-8937en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1021/acschembio.5b00502en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


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