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dc.contributor.authorDillon, Michael
dc.contributor.authorBowkett, AE
dc.contributor.authorBungard, MJ
dc.contributor.authorBeckman, KM
dc.contributor.authorO'Brien, MF
dc.contributor.authorBates, K
dc.contributor.authorFisher, MC
dc.contributor.authorStevens, JR
dc.contributor.authorThornton, CR
dc.date.accessioned2021-09-16T14:34:15Z
dc.date.available2021-09-16T14:34:15Z
dc.date.issued2017-03
dc.identifier.issn1751-7915
dc.identifier.issn1751-7915
dc.identifier.urihttp://hdl.handle.net/10026.1/17805
dc.description.abstract

The fungus Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a lethal epizootic disease of amphibians. Rapid identification of the pathogen and biosecurity is essential to prevent its spread, but current laboratory-based tests are time-consuming and require specialist equipment. Here, we describe the generation of an IgM monoclonal antibody (mAb), 5C4, specific to Bd as well as the related salamander and newt pathogen Batrachochytrium salamandrivorans (Bsal). The mAb, which binds to a glycoprotein antigen present on the surface of zoospores, sporangia and zoosporangia, was used to develop a lateral-flow assay (LFA) for rapid (15 min) detection of the pathogens. The LFA detects known lineages of Bd and also Bsal, as well as the closely related fungus Homolaphlyctis polyrhiza, but does not detect a wide range of related and unrelated fungi and oomycetes likely to be present in amphibian habitats. When combined with a simple swabbing procedure, the LFA was 100% accurate in detecting the water-soluble 5C4 antigen present in skin, foot and pelvic samples from frogs, newts and salamanders naturally infected with Bd or Bsal. Our results demonstrate the potential of the portable LFA as a rapid qualitative assay for tracking these amphibian pathogens and as an adjunct test to nucleic acid-based detection methods.

dc.format.extent381-394
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherWiley
dc.subjectAnimals
dc.subjectAntibodies, Fungal
dc.subjectAntibodies, Monoclonal
dc.subjectAntigens, Fungal
dc.subjectChromatography, Affinity
dc.subjectChytridiomycota
dc.subjectDiagnostic Tests, Routine
dc.subjectImmunoglobulin M
dc.subjectMycoses
dc.subjectUrodela
dc.subjectVeterinary Medicine
dc.titleTracking the amphibian pathogens <i>Batrachochytrium dendrobatidis</i> and <i>Batrachochytrium salamandrivorans</i> using a highly specific monoclonal antibody and lateral-flow technology
dc.typejournal-article
dc.typeEvaluation Study
dc.typeJournal Article
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000423629200016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue2
plymouth.volume10
plymouth.publication-statusPublished
plymouth.journalMicrobial Biotechnology
dc.identifier.doi10.1111/1751-7915.12464
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dcterms.dateAccepted2016-10-27
dc.rights.embargodate2023-8-9
dc.identifier.eissn1751-7915
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1111/1751-7915.12464
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2017-03
rioxxterms.typeJournal Article/Review


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