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dc.contributor.authorDillon, MJen
dc.contributor.authorBowkett, AEen
dc.contributor.authorBungard, MJen
dc.contributor.authorBeckman, KMen
dc.contributor.authorO'Brien, MFen
dc.contributor.authorBates, Ken
dc.contributor.authorFisher, MCen
dc.contributor.authorStevens, JRen
dc.contributor.authorThornton, CRen
dc.date.accessioned2021-09-16T14:34:15Z
dc.date.available2021-09-16T14:34:15Z
dc.date.issued2017-03en
dc.identifier.urihttp://hdl.handle.net/10026.1/17805
dc.description.abstract

The fungus Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a lethal epizootic disease of amphibians. Rapid identification of the pathogen and biosecurity is essential to prevent its spread, but current laboratory-based tests are time-consuming and require specialist equipment. Here, we describe the generation of an IgM monoclonal antibody (mAb), 5C4, specific to Bd as well as the related salamander and newt pathogen Batrachochytrium salamandrivorans (Bsal). The mAb, which binds to a glycoprotein antigen present on the surface of zoospores, sporangia and zoosporangia, was used to develop a lateral-flow assay (LFA) for rapid (15 min) detection of the pathogens. The LFA detects known lineages of Bd and also Bsal, as well as the closely related fungus Homolaphlyctis polyrhiza, but does not detect a wide range of related and unrelated fungi and oomycetes likely to be present in amphibian habitats. When combined with a simple swabbing procedure, the LFA was 100% accurate in detecting the water-soluble 5C4 antigen present in skin, foot and pelvic samples from frogs, newts and salamanders naturally infected with Bd or Bsal. Our results demonstrate the potential of the portable LFA as a rapid qualitative assay for tracking these amphibian pathogens and as an adjunct test to nucleic acid-based detection methods.

en
dc.format.extent381 - 394en
dc.languageengen
dc.language.isoengen
dc.subjectAnimalsen
dc.subjectAntibodies, Fungalen
dc.subjectAntibodies, Monoclonalen
dc.subjectAntigens, Fungalen
dc.subjectChromatography, Affinityen
dc.subjectChytridiomycotaen
dc.subjectDiagnostic Tests, Routineen
dc.subjectImmunoglobulin Men
dc.subjectMycosesen
dc.subjectUrodelaen
dc.subjectVeterinary Medicineen
dc.titleTracking the amphibian pathogens Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans using a highly specific monoclonal antibody and lateral-flow technology.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/27995742en
plymouth.issue2en
plymouth.volume10en
plymouth.publication-statusPublisheden
plymouth.journalMicrob Biotechnolen
dc.identifier.doi10.1111/1751-7915.12464en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited Statesen
dcterms.dateAccepted2016-10-27en
dc.identifier.eissn1751-7915en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1111/1751-7915.12464en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-03en
rioxxterms.typeJournal Article/Reviewen


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