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dc.contributor.authorVitale, GA
dc.contributor.authorSciarretta, M
dc.contributor.authorPalma Esposito, F
dc.contributor.authorJanuary, Grant
dc.contributor.authorGiaccio, M
dc.contributor.authorBunk, B
dc.contributor.authorSpröer, C
dc.contributor.authorBajerski, F
dc.contributor.authorPower, D
dc.contributor.authorFesta, C
dc.contributor.authorMonti, MC
dc.contributor.authorD’Auria, MV
dc.contributor.authorde Pascale, D
dc.date.accessioned2021-08-09T11:00:34Z
dc.date.available2021-08-09T11:00:34Z
dc.date.issued2020-05-22
dc.identifier.issn0163-3864
dc.identifier.issn1520-6025
dc.identifier.urihttp://hdl.handle.net/10026.1/17449
dc.description.abstract

A wide range of prescreening tests for antimicrobial activity of 59 bacterial isolates from sediments of Ria Formosa Lagoon (Algarve, Portugal) disclosed Vibrio spartinae 3.6 as the most active antibacterial producing strain. This bacterial strain, which has not previously been submitted for chemical profiling, was subjected to de novo whole genome sequencing, which aided in the discovery and elucidation of a prodigiosin biosynthetic gene cluster that was predicted by the bioinformatic tool KEGG BlastKoala. Comparative genomics led to the identification of a new membrane di-iron oxygenase-like enzyme, annotated as Vspart_02107, which is likely to be involved in the biosynthesis of cycloprodigiosin and analogues. The combined genomics-metabolomics profiling of the strain led to the isolation and identification of one new branched-chain prodigiosin (5) and to the detection of two new cyclic forms. Furthermore, the evaluation of the minimum inhibitory concentrations disclosed the major prodigiosin as very effective against multi-drug-resistant pathogens including Stenotrophomonas maltophilia, a clinical isolate of Listeria monocytogenes, as well as some human pathogens reported by the World Health Organization as prioritized targets.

dc.format.extent1495-1504
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)
dc.subjectAnti-Bacterial Agents
dc.subjectBacteria
dc.subjectComputational Biology
dc.subjectCyclization
dc.subjectDrug Resistance, Multiple, Bacterial
dc.subjectGenomics
dc.subjectIndoles
dc.subjectListeria monocytogenes
dc.subjectMetabolomics
dc.subjectMicrobial Sensitivity Tests
dc.subjectPyrroles
dc.subjectSpectrometry, Mass, Electrospray Ionization
dc.subjectStenotrophomonas maltophilia
dc.subjectVibrio
dc.titleGenomics–Metabolomics Profiling Disclosed Marine Vibrio spartinae 3.6 as a Producer of a New Branched Side Chain Prodigiosin
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000537539300017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue5
plymouth.volume83
plymouth.publication-statusPublished
plymouth.journalJournal of Natural Products
dc.identifier.doi10.1021/acs.jnatprod.9b01159
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dcterms.dateAccepted2020-03-14
dc.rights.embargodate2021-9-14
dc.identifier.eissn1520-6025
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1021/acs.jnatprod.9b01159
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-05-22
rioxxterms.typeJournal Article/Review


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