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dc.contributor.authorSafarzadeh, Men
dc.contributor.authorSuhail, Aen
dc.contributor.authorSethi, Jen
dc.contributor.authorSattar, Aen
dc.contributor.authorJenkins, Den
dc.contributor.authorPan, Gen
dc.date.accessioned2021-06-13T16:00:58Z
dc.date.issued2021-04-12en
dc.identifier.issn2079-4991en
dc.identifier.urihttp://hdl.handle.net/10026.1/17249
dc.description.abstract

<jats:p>In this work, we developed a sandwich DNA-immunosensor for quantification of the methylated tumour suppressor gene O-6-methylguanine-DNA methyltransferase (MGMT), which is a potential biomarker for brain tumours and breast cancer. The biosensor is based on aminated reduced graphene oxide electrode, which is achieved by ammonium hydroxide chemisorption and anti-5-methylcytosine (anti-5mC) as a methylation bioreceptor. The target single-strand (ss) MGMT oligonucleotide is first recognised by its hybridisation with complementary DNA to form double-stranded (ds) MGMT, which is then captured by anti-5mC on the electrode surface due to the presence of methylation. Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and Scanning electron microscopy (SEM) techniques were used to characterise the electrode surface. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used for electrochemical measurements. Under optimised conditions, the proposed biosensor is able to quantify a linear range of concentrations of the MGMT gene from 50 fM to 100 pM with a limit of detection (LOD) of 12 fM. The sandwich design facilitates the simultaneous recognition and quantification of DNA methylation, and the amination significantly improves the sensitivity of the biosensor. This biosensor is label-, bisulfite- and PCR-free and has a simple design for cost-efficient production. It can also be tailor-made to detect other methylated genes, which makes it a promising detection platform for DNA methylation-related disease diagnosis and prognosis.</jats:p>

en
dc.format.extent985 - 985en
dc.languageenen
dc.language.isoenen
dc.publisherMDPIen
dc.titleA Label-Free DNA-Immunosensor Based on Aminated rGO Electrode for the Quantification of DNA Methylationen
dc.typeJournal Article
plymouth.issue4en
plymouth.volume11en
plymouth.journalNanomaterialsen
dc.identifier.doi10.3390/nano11040985en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Engineering, Computing and Mathematics
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA12 Engineering
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
plymouth.organisational-group/Plymouth/Users by role/Post-Graduate Research Students
dcterms.dateAccepted2021-04-07en
dc.rights.embargodate2021-06-15en
dc.identifier.eissn2079-4991en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.3390/nano11040985en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2021-04-12en
rioxxterms.typeJournal Article/Reviewen


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