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dc.contributor.authorSofela, AA
dc.contributor.authorHilton, DA
dc.contributor.authorAmmoun, Sylwia
dc.contributor.authorBaiz, D
dc.contributor.authorAdams, Claire
dc.contributor.authorErcolano, E
dc.contributor.authorJenkinson, MD
dc.contributor.authorKurian, KM
dc.contributor.authorTeo, M
dc.contributor.authorWhitfield, Peter
dc.contributor.authorSahm, F
dc.contributor.authorHanemann, Clemens Oliver
dc.date.accessioned2021-03-25T12:21:00Z
dc.date.issued2021-01-08
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067
dc.identifier.otherARTN 560
dc.identifier.urihttp://hdl.handle.net/10026.1/16980
dc.description.abstract

<jats:p>There is an unmet need for the identification of biomarkers to aid in the diagnosis, clinical management, prognosis and follow-up of meningiomas. There is currently no consensus on the optimum management of WHO grade II meningiomas. In this study, we identified the calcium binding extracellular matrix glycoprotein, Fibulin-2, via mass-spectrometry-based proteomics, assessed its expression in grade I and II meningiomas and explored its potential as a grade II biomarker. A total of 87 grade I and 91 grade II different meningioma cells, tissue and plasma samples were used for the various experimental techniques employed to assess Fibulin-2 expression. The tumours were reviewed and classified according to the 2016 edition of the Classification of the Tumours of the central nervous system (CNS). Mass spectrometry proteomic analysis identified Fibulin-2 as a differentially expressed protein between grade I and II meningioma cell cultures. Fibulin-2 levels were further evaluated in meningioma cells using Western blotting and Real-time Quantitative Polymerase Chain Reaction (RT-qPCR); in meningioma tissues via immunohistochemistry and RT-qPCR; and in plasma via Enzyme-Linked Immunosorbent Assay (ELISA). Proteomic analyses (p &lt; 0.05), Western blotting (p &lt; 0.05) and RT-qPCR (p &lt; 0.01) confirmed significantly higher Fibulin-2 (FBLN2) expression levels in grade II meningiomas compared to grade I. Fibulin-2 blood plasma levels were also significantly higher in grade II meningioma patients compared to grade I patients. This study suggests that elevated Fibulin-2 might be a novel grade II meningioma biomarker, when differentiating them from the grade I tumours. The trend of Fibulin-2 expression observed in plasma may serve as a useful non-invasive biomarker.</jats:p>

dc.format.extent560-560
dc.format.mediumElectronic
dc.languageen
dc.language.isoen
dc.publisherMDPI AG
dc.subjectmeningioma
dc.subjectatypical
dc.subjectbenign
dc.subjectbiomarker
dc.subjectplasma
dc.titleFibulin-2: A Novel Biomarker for Differentiating Grade II from Grade I Meningiomas
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000611326600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue2
plymouth.volume22
plymouth.publication-statusPublished online
plymouth.journalInternational Journal of Molecular Sciences
dc.identifier.doi10.3390/ijms22020560
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR)
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
plymouth.organisational-group/Plymouth/Users by role/Researchers in ResearchFish submission
dc.publisher.placeSwitzerland
dcterms.dateAccepted2021-01-05
dc.rights.embargodate2021-3-27
dc.identifier.eissn1422-0067
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.3390/ijms22020560
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-01-08
rioxxterms.typeJournal Article/Review


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