Galleria mellonella larvae exhibit a weight-dependent lethal median dose when infected with methicillin-resistant Staphylococcus aureus
dc.contributor.author | Hesketh-Best, PJ | |
dc.contributor.author | Mouritzen, MV | |
dc.contributor.author | Shandley-Edwards, K | |
dc.contributor.author | Billington, Richard | |
dc.contributor.author | Upton, Mathew | |
dc.date.accessioned | 2021-02-17T09:09:22Z | |
dc.date.available | 2021-02-17T09:09:22Z | |
dc.date.issued | 2021-03 | |
dc.identifier.issn | 2049-632X | |
dc.identifier.issn | 2049-632X | |
dc.identifier.other | ftab003 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/16889 | |
dc.description.abstract |
<jats:title>ABSTRACT</jats:title> <jats:p>Galleria mellonella is a recognised model to study antimicrobial efficacy; however, standardisation across the scientific field and investigations of methodological components are needed. Here, we investigate the impact of weight on mortality following infection with Methicillin-resistant Staphylococcus aureus (MRSA). Larvae were separated into six weight groups (180–300 mg at 20 mg intervals) and infected with a range of doses of MRSA to determine the 50% lethal dose (LD50), and the ‘lipid weight’ of larvae post-infection was quantified. A model of LD50 values correlated with weight was developed. The LD50 values, as estimated by our model, were further tested in vivo to prove our model.</jats:p> <jats:p>We establish a weight-dependent LD50 in larvae against MRSA and demonstrate that G. mellonella is a stable model within 180–260 mg. We present multiple linear models correlating weight with: LD50, lipid weight, and larval length. We demonstrate that the lipid weight is reduced as a result of MRSA infection, identifying a potentially new measure in which to understand the immune response. Finally, we demonstrate that larval length can be a reasonable proxy for weight. Refining the methodologies in which to handle and design experiments involving G. mellonella, we can improve the reliability of this powerful model.</jats:p> | |
dc.format.extent | ftab003- | |
dc.format.medium | ||
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Oxford University Press (OUP) | |
dc.subject | Methicillin-resistant Staphylococcus aureus | |
dc.subject | Galleria mellonella | |
dc.subject | antibiotic testing | |
dc.subject | LD50 | |
dc.subject | pre-clinical model | |
dc.subject | fat body | |
dc.title | Galleria mellonella larvae exhibit a weight-dependent lethal median dose when infected with methicillin-resistant Staphylococcus aureus | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000637322900004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 2 | |
plymouth.volume | 79 | |
plymouth.publication-status | Published | |
plymouth.journal | Pathogens and Disease | |
dc.identifier.doi | 10.1093/femspd/ftab003 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA03 Allied Health Professions, Dentistry, Nursing and Pharmacy | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR) | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2021-01-11 | |
dc.rights.embargodate | 2021-12-3 | |
dc.identifier.eissn | 2049-632X | |
dc.rights.embargoperiod | Not known | |
rioxxterms.funder | Innovate UK | |
rioxxterms.identifier.project | A new pipeline of first in class antibiotics templated on the bacteriocins | |
rioxxterms.versionofrecord | 10.1093/femspd/ftab003 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2021-03 | |
rioxxterms.type | Journal Article/Review | |
plymouth.funder | A new pipeline of first in class antibiotics templated on the bacteriocins::Innovate UK | |
plymouth.funder | A new pipeline of first in class antibiotics templated on the bacteriocins::Innovate UK |