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dc.contributor.authorHesketh-Best, PJen
dc.contributor.authorMouritzen, MVen
dc.contributor.authorShandley-Edwards, Ken
dc.contributor.authorBillington, RAen
dc.contributor.authorUpton, Men
dc.date.accessioned2021-02-17T09:09:22Z
dc.date.available2021-02-17T09:09:22Z
dc.date.issued2021-03en
dc.identifier.otherftab003en
dc.identifier.urihttp://hdl.handle.net/10026.1/16889
dc.description.abstract

<jats:title>ABSTRACT</jats:title> <jats:p>Galleria mellonella is a recognised model to study antimicrobial efficacy; however, standardisation across the scientific field and investigations of methodological components are needed. Here, we investigate the impact of weight on mortality following infection with Methicillin-resistant Staphylococcus aureus (MRSA). Larvae were separated into six weight groups (180–300 mg at 20 mg intervals) and infected with a range of doses of MRSA to determine the 50% lethal dose (LD50), and the ‘lipid weight’ of larvae post-infection was quantified. A model of LD50 values correlated with weight was developed. The LD50 values, as estimated by our model, were further tested in vivo to prove our model.</jats:p> <jats:p>We establish a weight-dependent LD50 in larvae against MRSA and demonstrate that G. mellonella is a stable model within 180–260 mg. We present multiple linear models correlating weight with: LD50, lipid weight, and larval length. We demonstrate that the lipid weight is reduced as a result of MRSA infection, identifying a potentially new measure in which to understand the immune response. Finally, we demonstrate that larval length can be a reasonable proxy for weight. Refining the methodologies in which to handle and design experiments involving G. mellonella, we can improve the reliability of this powerful model.</jats:p>

en
dc.languageenen
dc.language.isoenen
dc.publisherOxford University Press (OUP)en
dc.titleGalleria mellonella larvae exhibit a weight-dependent lethal median dose when infected with methicillin-resistant Staphylococcus aureusen
dc.typeJournal Article
plymouth.issue2en
plymouth.volume79en
plymouth.journalPathogens and Diseaseen
dc.identifier.doi10.1093/femspd/ftab003en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA03 Allied Health Professions, Dentistry, Nursing and Pharmacy
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dcterms.dateAccepted2021-01-11en
dc.rights.embargodate2022-01-27en
dc.identifier.eissn2049-632Xen
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1093/femspd/ftab003en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2021-03en
rioxxterms.typeJournal Article/Reviewen


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