Show simple item record

dc.contributor.authorRawling, Mark
dc.contributor.authorLeclercq, E
dc.contributor.authorFoey, Andrew
dc.contributor.authorCastex, M
dc.contributor.authorMerrifield, Daniel
dc.date.accessioned2021-01-13T10:12:28Z
dc.date.issued2021-01-12
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.otherARTN e0245021
dc.identifier.urihttp://hdl.handle.net/10026.1/16799
dc.description.abstract

<jats:p>This study was conducted to evaluate the mucosal immune responses of rainbow trout when supplementing an experimental formulated feed with multi-strain yeast fraction product (<jats:italic>Saccharomyces cerevisiae</jats:italic> and <jats:italic>Cyberlindnera jardinii</jats:italic>). In total, 360 fish (initial BW 23.1 ± 0.2 g) were randomly allotted into three dietary treatments in an 8-week feeding trial. The dietary treatments included basal diet (control) and control + 1.5 g/kg multi-strain yeast fraction product (MsYF) fed continuously and pulsed every two weeks between control and MsYF diet. No negative effects on growth performance of feeding the MsYF supplemented diet were observed. SGR and FCR averaged 2.30 ± 0.03%/day and 1.03 ± 0.03, respectively, across experimental groups. Muscularis thickness in the anterior intestine after 8 weeks of feeding was significantly elevated by 44.3% in fish fed the MsYF continuously, and by 14.4% in fish fed the MsYF pulsed (<jats:italic>P</jats:italic> &lt; 0.02). Significant elevations in goblet cell density in the anterior and posterior (&gt;50% increase) intestine were observed after 8 weeks of feeding the MsYF supplemented diet (<jats:italic>P</jats:italic>&lt; 0.03). In contrast, lamina propria width was significantly lower in fish fed the experimental diets (&gt;10% reduction). The gene expression analysis of the intestine revealed significant elevations in expression of <jats:italic>tlr2</jats:italic>, <jats:italic>il1r1</jats:italic>, <jats:italic>irak4</jats:italic>, and <jats:italic>tollip2</jats:italic> after 4 weeks of feeding the MsYF. Significant elevations in effector cytokines <jats:italic>tnfα</jats:italic>, <jats:italic>il10</jats:italic> and <jats:italic>tgfβ</jats:italic> were observed after 4 weeks of feeding the MsYF regime. After 8 weeks significant elevations in the gene expression levels of <jats:italic>il1β</jats:italic>, <jats:italic>ifnγ</jats:italic>, and <jats:italic>il12</jats:italic> were observed in fish fed the MsYF. Likewise, the expression of the transcription factor <jats:italic>gata3</jats:italic> was significantly elevated (<jats:italic>P</jats:italic>&lt;0.01). Supplementation of the multi-strain yeast fraction product positively modulates the intestinal mucosal response of rainbow trout through interaction with toll-like receptor two signalling pathway and potential for increased capacity of delivery of antigens to the underlying mucosal associated lymphoid tissue.</jats:p>

dc.format.extente0245021-e0245021
dc.format.mediumElectronic-eCollection
dc.languageen
dc.language.isoen
dc.publisherPublic Library of Science
dc.subjectAnimals
dc.subjectCytokines
dc.subjectDiet
dc.subjectImmunity, Mucosal
dc.subjectIntestinal Mucosa
dc.subjectOncorhynchus mykiss
dc.subjectSignal Transduction
dc.subjectToll-Like Receptors
dc.titleA novel dietary multi-strain yeast fraction modulates intestinal toll-like-receptor signalling and mucosal responses of rainbow trout (Oncorhynchus mykiss)
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000609979700030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue1
plymouth.volume16
plymouth.publication-statusPublished online
plymouth.journalPLoS One
dc.identifier.doi10.1371/journal.pone.0245021
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
plymouth.organisational-group/Plymouth/Users by role/Researchers in ResearchFish submission
dc.publisher.placeUnited States
dcterms.dateAccepted2020-12-19
dc.rights.embargodate2021-1-15
dc.identifier.eissn1932-6203
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1371/journal.pone.0245021
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-01-12
rioxxterms.typeJournal Article/Review


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
Atmire NV