A novel dietary multi-strain yeast fraction modulates intestinal toll-like-receptor signalling and mucosal responses of rainbow trout (Oncorhynchus mykiss)
dc.contributor.author | Rawling, Mark | |
dc.contributor.author | Leclercq, E | |
dc.contributor.author | Foey, Andrew | |
dc.contributor.author | Castex, M | |
dc.contributor.author | Merrifield, Daniel | |
dc.date.accessioned | 2021-01-13T10:12:28Z | |
dc.date.issued | 2021-01-12 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | ARTN e0245021 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/16799 | |
dc.description.abstract |
<jats:p>This study was conducted to evaluate the mucosal immune responses of rainbow trout when supplementing an experimental formulated feed with multi-strain yeast fraction product (<jats:italic>Saccharomyces cerevisiae</jats:italic> and <jats:italic>Cyberlindnera jardinii</jats:italic>). In total, 360 fish (initial BW 23.1 ± 0.2 g) were randomly allotted into three dietary treatments in an 8-week feeding trial. The dietary treatments included basal diet (control) and control + 1.5 g/kg multi-strain yeast fraction product (MsYF) fed continuously and pulsed every two weeks between control and MsYF diet. No negative effects on growth performance of feeding the MsYF supplemented diet were observed. SGR and FCR averaged 2.30 ± 0.03%/day and 1.03 ± 0.03, respectively, across experimental groups. Muscularis thickness in the anterior intestine after 8 weeks of feeding was significantly elevated by 44.3% in fish fed the MsYF continuously, and by 14.4% in fish fed the MsYF pulsed (<jats:italic>P</jats:italic> < 0.02). Significant elevations in goblet cell density in the anterior and posterior (>50% increase) intestine were observed after 8 weeks of feeding the MsYF supplemented diet (<jats:italic>P</jats:italic>< 0.03). In contrast, lamina propria width was significantly lower in fish fed the experimental diets (>10% reduction). The gene expression analysis of the intestine revealed significant elevations in expression of <jats:italic>tlr2</jats:italic>, <jats:italic>il1r1</jats:italic>, <jats:italic>irak4</jats:italic>, and <jats:italic>tollip2</jats:italic> after 4 weeks of feeding the MsYF. Significant elevations in effector cytokines <jats:italic>tnfα</jats:italic>, <jats:italic>il10</jats:italic> and <jats:italic>tgfβ</jats:italic> were observed after 4 weeks of feeding the MsYF regime. After 8 weeks significant elevations in the gene expression levels of <jats:italic>il1β</jats:italic>, <jats:italic>ifnγ</jats:italic>, and <jats:italic>il12</jats:italic> were observed in fish fed the MsYF. Likewise, the expression of the transcription factor <jats:italic>gata3</jats:italic> was significantly elevated (<jats:italic>P</jats:italic><0.01). Supplementation of the multi-strain yeast fraction product positively modulates the intestinal mucosal response of rainbow trout through interaction with toll-like receptor two signalling pathway and potential for increased capacity of delivery of antigens to the underlying mucosal associated lymphoid tissue.</jats:p> | |
dc.format.extent | e0245021-e0245021 | |
dc.format.medium | Electronic-eCollection | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Public Library of Science | |
dc.subject | Animals | |
dc.subject | Cytokines | |
dc.subject | Diet | |
dc.subject | Immunity, Mucosal | |
dc.subject | Intestinal Mucosa | |
dc.subject | Oncorhynchus mykiss | |
dc.subject | Signal Transduction | |
dc.subject | Toll-Like Receptors | |
dc.title | A novel dietary multi-strain yeast fraction modulates intestinal toll-like-receptor signalling and mucosal responses of rainbow trout (Oncorhynchus mykiss) | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000609979700030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 1 | |
plymouth.volume | 16 | |
plymouth.publication-status | Published online | |
plymouth.journal | PLoS One | |
dc.identifier.doi | 10.1371/journal.pone.0245021 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2020-12-19 | |
dc.rights.embargodate | 2021-1-15 | |
dc.identifier.eissn | 1932-6203 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1371/journal.pone.0245021 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2021-01-12 | |
rioxxterms.type | Journal Article/Review |