Effect of L-Methionine Feeding on Serum Homocysteine and Glutathione Levels in Male and Female Wistar Rats
dc.contributor.author | Ahmad Demerchi, S | |
dc.contributor.author | Robert McFarlane, J | |
dc.contributor.author | Dominique Jean Moens, P | |
dc.contributor.author | King, N | |
dc.date.accessioned | 2020-09-28T10:25:39Z | |
dc.date.available | 2020-09-28T10:25:39Z | |
dc.date.issued | 2020-03-23 | |
dc.identifier.issn | 2329-0870 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/16433 | |
dc.description.abstract |
Homocysteine (Hcy) is a critical indicator of cardiovascular disease. High levels of Hcy have now been recognised as a risk factor for the development of a wide range of diseases. Hyperhomocysteinemia (Hhcy) can be induced by methionine or Hcy supplementation. On the other hand, Glutathione (GSH) is a major antioxidant in the body and also an important compound for oxidative defence. It is composed of 3 amino acids: cysteine, glutamate, and glycine. Interestingly, methionine is also a crucial compound in GSH synthesis. This study aims to assess the impact of 1% L-methionine feeding (10 or 30 weeks) on the body weight and serum Hcy and GSH levels of young adult (16 weeks) and middle-aged (36 weeks) Wistar rats of both sexes. Serum was analysed for Hcy and reduced GSH levels by liquid chromatography mass spectrometry (LCMS) in response to 1% L-methionine feeding. One percent L-methionine feeding decreased body weight in all conditions investigated, although this only reached significance in males after 10 weeks supplementation and females after 30 weeks supplementation. It also induced a significant increase in the serum Hcy levels of male Wistar rats, whilst having no significant effect on Hcy serum levels in female rats. Finally, we also observed a small increase in serum GSH levels in female Wistar rats but no change in serum GSH levels in the males. These results suggest that methionine feeding affects body weight homeostasis and alters by products of methionine catabolism. | |
dc.format.extent | 21-21 | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Science Publishing Group | |
dc.title | Effect of L-Methionine Feeding on Serum Homocysteine and Glutathione Levels in Male and Female Wistar Rats | |
dc.type | journal-article | |
plymouth.issue | 1 | |
plymouth.volume | 8 | |
plymouth.publication-status | Published | |
plymouth.journal | Advances in Biochemistry | |
dc.identifier.doi | 10.11648/j.ab.20200801.14 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA03 Allied Health Professions, Dentistry, Nursing and Pharmacy | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dcterms.dateAccepted | 2020-03-10 | |
dc.rights.embargodate | 2020-9-29 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.11648/j.ab.20200801.14 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2020-03-23 | |
rioxxterms.type | Journal Article/Review |