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dc.contributor.authorTillack, K
dc.contributor.authorAboutalebi, H
dc.contributor.authorKramer, Edgar
dc.date.accessioned2020-09-15T13:39:54Z
dc.date.available2020-09-15T13:39:54Z
dc.date.issued2015-08-20
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.otherARTN e0136203
dc.identifier.urihttp://hdl.handle.net/10026.1/16351
dc.description.abstract

BACKGROUND & AIMS: The brain dopaminergic (DA) system is involved in fine tuning many behaviors and several human diseases are associated with pathological alterations of the DA system such as Parkinson's disease (PD) and drug addiction. Because of its complex network integration, detailed analyses of physiological and pathophysiological conditions are only possible in a whole organism with a sophisticated tool box for visualization and functional modification. METHODS & RESULTS: Here, we have generated transgenic mice expressing the tetracycline-regulated transactivator (tTA) or the reverse tetracycline-regulated transactivator (rtTA) under control of the tyrosine hydroxylase (TH) promoter, TH-tTA (tet-OFF) and TH-rtTA (tet-ON) mice, to visualize and genetically modify DA neurons. We show their tight regulation and efficient use to overexpress proteins under the control of tet-responsive elements or to delete genes of interest with tet-responsive Cre. In combination with mice encoding tet-responsive luciferase, we visualized the DA system in living mice progressively over time. CONCLUSION: These experiments establish TH-tTA and TH-rtTA mice as a powerful tool to generate and monitor mouse models for DA system diseases.

dc.format.extente0136203-e0136203
dc.format.mediumElectronic-eCollection
dc.languageen
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.subjectAnimals
dc.subjectDisease Models, Animal
dc.subjectDopaminergic Neurons
dc.subjectGene Expression
dc.subjectGene Expression Regulation
dc.subjectGene Targeting
dc.subjectGenetic Vectors
dc.subjectHumans
dc.subjectLuminescent Measurements
dc.subjectMice
dc.subjectMice, Transgenic
dc.subjectOptical Imaging
dc.subjectPromoter Regions, Genetic
dc.subjectTetracycline
dc.subjectTrans-Activators
dc.subjectTyrosine 3-Monooxygenase
dc.titleAn Efficient and Versatile System for Visualization and Genetic Modification of Dopaminergic Neurons in Transgenic Mice
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000359919900064&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue8
plymouth.volume10
plymouth.publication-statusPublished online
plymouth.journalPLOS ONE
dc.identifier.doi10.1371/journal.pone.0136203
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dcterms.dateAccepted2015-07-30
dc.identifier.eissn1932-6203
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1371/journal.pone.0136203
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015
rioxxterms.typeJournal Article/Review


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