An Efficient and Versatile System for Visualization and Genetic Modification of Dopaminergic Neurons in Transgenic Mice
dc.contributor.author | Tillack, K | |
dc.contributor.author | Aboutalebi, H | |
dc.contributor.author | Kramer, Edgar | |
dc.date.accessioned | 2020-09-15T13:39:54Z | |
dc.date.available | 2020-09-15T13:39:54Z | |
dc.date.issued | 2015-08-20 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | ARTN e0136203 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/16351 | |
dc.description.abstract |
BACKGROUND & AIMS: The brain dopaminergic (DA) system is involved in fine tuning many behaviors and several human diseases are associated with pathological alterations of the DA system such as Parkinson's disease (PD) and drug addiction. Because of its complex network integration, detailed analyses of physiological and pathophysiological conditions are only possible in a whole organism with a sophisticated tool box for visualization and functional modification. METHODS & RESULTS: Here, we have generated transgenic mice expressing the tetracycline-regulated transactivator (tTA) or the reverse tetracycline-regulated transactivator (rtTA) under control of the tyrosine hydroxylase (TH) promoter, TH-tTA (tet-OFF) and TH-rtTA (tet-ON) mice, to visualize and genetically modify DA neurons. We show their tight regulation and efficient use to overexpress proteins under the control of tet-responsive elements or to delete genes of interest with tet-responsive Cre. In combination with mice encoding tet-responsive luciferase, we visualized the DA system in living mice progressively over time. CONCLUSION: These experiments establish TH-tTA and TH-rtTA mice as a powerful tool to generate and monitor mouse models for DA system diseases. | |
dc.format.extent | e0136203-e0136203 | |
dc.format.medium | Electronic-eCollection | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Public Library of Science (PLoS) | |
dc.subject | Animals | |
dc.subject | Disease Models, Animal | |
dc.subject | Dopaminergic Neurons | |
dc.subject | Gene Expression | |
dc.subject | Gene Expression Regulation | |
dc.subject | Gene Targeting | |
dc.subject | Genetic Vectors | |
dc.subject | Humans | |
dc.subject | Luminescent Measurements | |
dc.subject | Mice | |
dc.subject | Mice, Transgenic | |
dc.subject | Optical Imaging | |
dc.subject | Promoter Regions, Genetic | |
dc.subject | Tetracycline | |
dc.subject | Trans-Activators | |
dc.subject | Tyrosine 3-Monooxygenase | |
dc.title | An Efficient and Versatile System for Visualization and Genetic Modification of Dopaminergic Neurons in Transgenic Mice | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000359919900064&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 8 | |
plymouth.volume | 10 | |
plymouth.publication-status | Published online | |
plymouth.journal | PLOS ONE | |
dc.identifier.doi | 10.1371/journal.pone.0136203 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/Peninsula Medical School | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2015-07-30 | |
dc.identifier.eissn | 1932-6203 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1371/journal.pone.0136203 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2015 | |
rioxxterms.type | Journal Article/Review |