Flowering poration – a synergistic multi-mode antibacterial mechanism by a bacteriocin fold
dc.contributor.author | Hammond, K | |
dc.contributor.author | Lewis, H | |
dc.contributor.author | Halliwell, S | |
dc.contributor.author | Desriac, F | |
dc.contributor.author | Nardone, B | |
dc.contributor.author | Ravi, J | |
dc.contributor.author | Hoogenboom, BW | |
dc.contributor.author | Upton, Mathew | |
dc.contributor.author | Derrick, JP | |
dc.contributor.author | Ryadnov, MG | |
dc.date.accessioned | 2020-08-09T10:14:23Z | |
dc.date.available | 2020-08-09T10:14:23Z | |
dc.date.issued | 2020-07-29 | |
dc.identifier.issn | 2589-0042 | |
dc.identifier.issn | 2589-0042 | |
dc.identifier.other | 101423 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/16145 | |
dc.description.abstract |
Bacteriocins are a distinct family of antimicrobial proteins postulated to porate bacterial membranes. However, direct experimental evidence of pore formation by these proteins is lacking. Here we report a multi-mode poration mechanism induced by four-helix bacteriocins, epidermicin NI01 and aureocin A53. Using a combination of crystallography, spectroscopy, bioassays, and nanoscale imaging, we established that individual two-helix segments of epidermicin retain antibacterial activity but each of these segments adopts a particular poration mode. In the intact protein these segments act synergistically to balance out antibacterial and hemolytic activities. The study sets a precedent of multi-mode membrane disruption advancing the current understanding of structure-activity relationships in pore-forming proteins. | |
dc.format.extent | 101423-101423 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Elsevier BV | |
dc.subject | Biological Sciences | |
dc.subject | Microbiology | |
dc.subject | Structural Biology | |
dc.title | Flowering poration – a synergistic multi-mode antibacterial mechanism by a bacteriocin fold | |
dc.type | journal-article | |
dc.type | Journal Article | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000564160300002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 8 | |
plymouth.volume | 23 | |
plymouth.publication-status | Published | |
plymouth.journal | iScience | |
dc.identifier.doi | 10.1016/j.isci.2020.101423 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR) | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2020-07-27 | |
dc.rights.embargodate | 2020-8-13 | |
dc.identifier.eissn | 2589-0042 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.funder | Innovate UK | |
rioxxterms.identifier.project | A new pipeline of first in class antibiotics templated on the bacteriocins | |
rioxxterms.versionofrecord | 10.1016/j.isci.2020.101423 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2020-07-29 | |
rioxxterms.type | Journal Article/Review | |
plymouth.funder | A new pipeline of first in class antibiotics templated on the bacteriocins::Innovate UK | |
plymouth.funder | A new pipeline of first in class antibiotics templated on the bacteriocins::Innovate UK | |
plymouth.funder | A new pipeline of first in class antibiotics templated on the bacteriocins::Innovate UK |