Coastal bacterioplankton community response to diatom-derived polysaccharide microgels
dc.contributor.author | Taylor, JD | |
dc.contributor.author | Cunliffe, Michael | |
dc.date.accessioned | 2020-07-16T07:59:45Z | |
dc.date.available | 2020-07-16T07:59:45Z | |
dc.date.issued | 2017-04 | |
dc.identifier.issn | 1758-2229 | |
dc.identifier.issn | 1758-2229 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/16045 | |
dc.description.abstract |
<jats:title>Summary</jats:title><jats:p>Phytoplankton‐derived polysaccharide microgels, including transparent exopolymer particles (TEP), are a major component of the marine organic carbon pool. Previous studies have made correlative links between phytoplankton material and bacterioplankton, and performed experiments that assess general responses to phytoplankton, yet there is a lack of direct empirical evidence of specific bacterioplankton responses to natural phytoplankton polysaccharide microgels. In this study, we used diatom produced TEP in controlled incubation experiments to determine the impact of polysaccharide microgels on a coastal bacterioplankton community. Quantification of bacterial 16S rRNA gene transcripts showed that the addition of TEP caused an increase in bacterioplankton activity. Similarly, high‐throughput sequencing of RT‐PCR amplified bacterial 16S rRNA gene transcripts showed that active bacterioplankton community structure and diversity also changed in response to microgels. Alteromonadales and Rhodobacterales increased in abundance in response to TEP, suggesting that both bacterioplankton taxa utilize diatom‐derived microgels. However, through assessing <jats:sup>13</jats:sup>C‐labelled TEP uptake via RNA Stable Isotope Probing, we show that only the Alteromonadales (genus <jats:italic>Alteromonas</jats:italic>) assimilated the TEP carbon. This study adds utilization of diatom‐derived TEP to the metabolic repertoire of the archetypal copiotrophic bacterioplankton <jats:italic>Alteromonas</jats:italic>, and indicates that the Rhodobacterales may utilize TEP for other purposes (e.g. attachment sites).</jats:p> | |
dc.format.extent | 151-157 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | Wiley | |
dc.subject | Bacteria | |
dc.subject | Biota | |
dc.subject | DNA, Bacterial | |
dc.subject | DNA, Ribosomal | |
dc.subject | Diatoms | |
dc.subject | Environmental Microbiology | |
dc.subject | Phylogeny | |
dc.subject | Polysaccharides | |
dc.subject | RNA, Ribosomal, 16S | |
dc.subject | Sequence Analysis, DNA | |
dc.title | Coastal bacterioplankton community response to diatom-derived polysaccharide microgels | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000397493300011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 2 | |
plymouth.volume | 9 | |
plymouth.publication-status | Published | |
plymouth.journal | Environmental Microbiology Reports | |
dc.identifier.doi | 10.1111/1758-2229.12513 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA07 Earth Systems and Environmental Sciences | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2016-12-05 | |
dc.identifier.eissn | 1758-2229 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1111/1758-2229.12513 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2017-04 | |
rioxxterms.type | Journal Article/Review |