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dc.contributor.authorTaylor, JD
dc.contributor.authorCunliffe, Michael
dc.date.accessioned2020-07-16T07:59:45Z
dc.date.available2020-07-16T07:59:45Z
dc.date.issued2017-04
dc.identifier.issn1758-2229
dc.identifier.issn1758-2229
dc.identifier.urihttp://hdl.handle.net/10026.1/16045
dc.description.abstract

<jats:title>Summary</jats:title><jats:p>Phytoplankton‐derived polysaccharide microgels, including transparent exopolymer particles (TEP), are a major component of the marine organic carbon pool. Previous studies have made correlative links between phytoplankton material and bacterioplankton, and performed experiments that assess general responses to phytoplankton, yet there is a lack of direct empirical evidence of specific bacterioplankton responses to natural phytoplankton polysaccharide microgels. In this study, we used diatom produced TEP in controlled incubation experiments to determine the impact of polysaccharide microgels on a coastal bacterioplankton community. Quantification of bacterial 16S rRNA gene transcripts showed that the addition of TEP caused an increase in bacterioplankton activity. Similarly, high‐throughput sequencing of RT‐PCR amplified bacterial 16S rRNA gene transcripts showed that active bacterioplankton community structure and diversity also changed in response to microgels. Alteromonadales and Rhodobacterales increased in abundance in response to TEP, suggesting that both bacterioplankton taxa utilize diatom‐derived microgels. However, through assessing <jats:sup>13</jats:sup>C‐labelled TEP uptake via RNA Stable Isotope Probing, we show that only the Alteromonadales (genus <jats:italic>Alteromonas</jats:italic>) assimilated the TEP carbon. This study adds utilization of diatom‐derived TEP to the metabolic repertoire of the archetypal copiotrophic bacterioplankton <jats:italic>Alteromonas</jats:italic>, and indicates that the Rhodobacterales may utilize TEP for other purposes (e.g. attachment sites).</jats:p>

dc.format.extent151-157
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherWiley
dc.subjectBacteria
dc.subjectBiota
dc.subjectDNA, Bacterial
dc.subjectDNA, Ribosomal
dc.subjectDiatoms
dc.subjectEnvironmental Microbiology
dc.subjectPhylogeny
dc.subjectPolysaccharides
dc.subjectRNA, Ribosomal, 16S
dc.subjectSequence Analysis, DNA
dc.titleCoastal bacterioplankton community response to diatom-derived polysaccharide microgels
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000397493300011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue2
plymouth.volume9
plymouth.publication-statusPublished
plymouth.journalEnvironmental Microbiology Reports
dc.identifier.doi10.1111/1758-2229.12513
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA07 Earth Systems and Environmental Sciences
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dcterms.dateAccepted2016-12-05
dc.identifier.eissn1758-2229
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1111/1758-2229.12513
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2017-04
rioxxterms.typeJournal Article/Review


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