Purification and characterization of nisin P produced by a strain of Streptococcus gallolyticus
Date
2020-03-03Author
Subject
Metadata
Show full item recordAbstract
<jats:p> <jats:bold>Introduction.</jats:bold> Against the backdrop of increasing resistance to conventional antibiotics, bacteriocins represent an attractive alternative, given their potent activity, novel modes of action and perceived lack of issues with resistance.</jats:p> <jats:p> <jats:bold>Aim.</jats:bold> In this study, the nature of the antibacterial activity of a clinical isolate of <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.11059" xlink:type="simple">Streptococcus gallolyticus</jats:ext-link> </jats:named-content> </jats:italic> was investigated.</jats:p> <jats:p> <jats:bold>Methods.</jats:bold> Optimization of the production of an inhibitor from strain AB39 was performed using different broth media and supplements. Purification was carried out using size exclusion, ion exchange and HPLC. Gel diffusion agar overlay, MS/MS, <jats:italic>de novo</jats:italic> peptide sequencing and genome mining were used in a proteogenomics approach to facilitate identification of the genetic basis for production of the inhibitor.</jats:p> <jats:p> <jats:bold>Results.</jats:bold> Strain AB39 was identified as representing <jats:italic> <jats:named-content content-type="subspecies"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5642" xlink:type="simple">Streptococcus gallolyticus</jats:ext-link> </jats:named-content> </jats:italic> subsp. <jats:italic> <jats:named-content content-type="subspecies"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5642" xlink:type="simple">pasteurianus</jats:ext-link> </jats:named-content> </jats:italic> and the successful production and purification of the AB39 peptide, named nisin P, with a mass of 3133.78 Da, was achieved using BHI broth with 10 % serum. Nisin P showed antibacterial activity towards clinical isolates of drug-resistant bacteria, including methicillin-resistant <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.11043" xlink:type="simple">Staphylococcus aureus</jats:ext-link> </jats:named-content> </jats:italic>, vancomycin-resistant <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5525" xlink:type="simple">Enterococcus</jats:ext-link> </jats:named-content> </jats:italic> and penicillin-resistant <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5677" xlink:type="simple">Streptococcus pneumoniae</jats:ext-link> </jats:named-content> </jats:italic>. In addition, the peptide exhibited significant stability towards high temperature, wide pH and certain proteolytic enzymes and displayed very low toxicity towards sheep red blood cells and Vero cells.</jats:p> <jats:p> <jats:bold>Conclusion.</jats:bold> To the best of our knowledge, this study represents the first production, purification and characterization of nisin P. Further study of nisin P may reveal its potential for treating or preventing infections caused by antibiotic-resistant Gram-positive bacteria, or those evading vaccination regimens.</jats:p>
Description
Collections
Publisher
Place of Publication
Journal
Volume
Issue
Pagination
Recommended, similar items
The following license files are associated with this item: