Use of compound specific δ13C stable isotope analysis for intact triacylglcerides using high temperature gas chromatography up to 430°C.
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We report a preliminary study demonstrating that high temperature gas chromatography with the oven operated at up to 430°C can be coupled with compound specific isotope ratio mass spectrometry (HTGC-CSIRMS) for the measurement of stable carbon isotopes. There was good agreement between data obtained using bulk and compound specific techniques for the C87 hydrocarbon apolane-87 and tripalmitolein. 13C values of three main triacylglycerides in different olive oils were measured for the first time and differed from bulk values of the olive oils. The use of HTGC-CSIRMS has wide-ranging application and warrants further study and validation.
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