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dc.contributor.authorAwan, Shakil
dc.date.accessioned2019-10-21T14:46:49Z
dc.date.issued2019-09-23
dc.identifier.urihttp://hdl.handle.net/10026.1/14995
dc.descriptionWorldwide prevalence of dementia is estimated to rise from the current 50 million people affected to 152 million by 2050, costing healthcare systems ~2% of the global GDP to diagnose, treat and care for patients [1]. Although there are over 130 different types of dementia, Alzheimer’s disease (AD) accounts for 60-70% of all cases. Methods for early diagnosis of AD could significantly impact disease detection, progression monitoring and therapeutics. This grand challenge requires ultra-sensitive and specific sensors to detect concentration level changes in patients significantly earlier than current diagnostic techniques employing MRI, CT, PET scans, blood tests and memory tests, often taking around 2 years to complete. We have therefore developed novel graphene/hBN transducers for the ultra-sensitive detection of AD protein biomarkers, such as Clusterin a molecular chaperone associated with AD, using electrical Quantum Transport Admittance Spectroscopy (e-QTAS). The graphene/hBN [2] transducers were fabricated on Si/SiO2 substrate using photolithography with evaporated chromium and sputtered gold contacts. The transducer channels were functionalized with linker molecules, 1-Pyrenebutyric acid N-hydroxysuccinimide (Pyr-NHS) ester, to immobile antiClusterin antibody (Ab) [3]. Binding reaction of the antibody with varying concentration levels of Clusterin antigen demonstrated the limit of detection of the transducers to be better than 10 fg/mL using four-probe direct current-voltage (DC-IV) and e-QTAS [4,5]. The developed transducers are generic, selective, fast, low-cost and could find applications in a broad range of point-of-care medical diagnostics in addition to neurodegenerative diseases (Alzheimer’s, Parkinson’s, etc.), such as cancer and cardiovascular disorders. We acknowledge funding from the UK’s EPSRC, EP/M006301/1 and University of Plymouth, GD105227.
dc.language.isoen
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleUltra-sensitive detection of Alzheimer’s protein biomarker Clusterin using novel Graphene/hBN transducers and Electrical Quantum Transport Admittance Spectroscopy (e-QTAS)
dc.typepresentation
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA12 Engineering
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.rights.embargodate2019-11-6
dc.rights.embargoperiodNot known
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
rioxxterms.typeOther


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