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dc.contributor.authorRodríguez-Rojas, Fen
dc.contributor.authorCelis-Plá, PSMen
dc.contributor.authorMéndez, Len
dc.contributor.authorMoenne, Fen
dc.contributor.authorMuñoz, PTen
dc.contributor.authorLobos, MGen
dc.contributor.authorDíaz, Pen
dc.contributor.authorSánchez-Lizaso, JLen
dc.contributor.authorBrown, MTen
dc.contributor.authorMoenne, Aen
dc.contributor.authorSáez, CAen
dc.date.accessioned2019-09-27T12:25:08Z
dc.date.available2019-09-27T12:25:08Z
dc.date.issued2019-09-13en
dc.identifier.issn1422-0067en
dc.identifier.urihttp://hdl.handle.net/10026.1/14941
dc.descriptionNo embargo required.en
dc.description.abstract

<jats:p>Following the physiological complementary/parallel Celis-Plá et al., by inhibiting extracellular signal regulated kinases (ERK), c-Jun N-terminal kinases (JNK), and cytokinin specific binding protein (p38), we assessed the role of the mitogen-activated protein kinases (MAPK) pathway in detoxification responses mediated by chronic copper (10 µM) in U. compressa. Parameters were taken at 6, 24, and 48 h, and 6 days (d). H2O2 and lipid peroxidation under copper and inhibition of ERK, JNK, or p38 alone increased but recovered by the sixth day. By blocking two or more MAPKs under copper, H2O2 and lipid peroxidation decayed even below controls. Inhibition of more than one MAPK (at 6 d) caused a decrease in total glutathione (reduced glutathione (GSH) + oxidised glutathione (GSSG)) and ascorbate (reduced ascorbate (ASC) + dehydroascorbate (DHA)), although in the latter it did not occur when the whole MAPK was blocked. Catalase (CAT), superoxide dismutase (SOD), thioredoxin (TRX) ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), and glutathione synthase (GS), were downregulated when blocking more than one MAPK pathway. When one MAPK pathway was blocked under copper, a recovery and even enhancement of detoxification mechanisms was observed, likely due to crosstalk within the MAPKs and/or other signalling processes. In contrast, when more than one MAPK pathway were blocked under copper, impairment of detoxification defences occurred, demonstrating that MAPKs were key signalling mechanisms for detoxification in macroalgae.</jats:p>

en
dc.format.extent4546 - 4546en
dc.languageenen
dc.language.isoenen
dc.publisherMDPIen
dc.titleMAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Involvement in the Regulation of Detoxification Mechanismsen
dc.typeJournal Article
plymouth.issue18en
plymouth.volume20en
plymouth.journalInternational Journal of Molecular Sciencesen
dc.identifier.doi10.3390/ijms20184546en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Marine Institute
dcterms.dateAccepted2019-09-01en
dc.rights.embargodate2019-11-26en
dc.identifier.eissn1422-0067en
dc.rights.embargoperiodNot knownen
rioxxterms.versionVoRen
rioxxterms.versionofrecord10.3390/ijms20184546en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-09-13en
rioxxterms.typeJournal Article/Reviewen


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