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dc.contributor.authorZhang, N
dc.contributor.authorThompson, CEL
dc.contributor.authorTownend, IH
dc.contributor.authorRankin, KE
dc.contributor.authorPaterson, DM
dc.contributor.authorManning, Andrew
dc.date.accessioned2019-01-21T18:15:46Z
dc.date.available2019-01-21T18:15:46Z
dc.date.issued2018-11-20
dc.identifier.issn0013-936X
dc.identifier.issn1520-5851
dc.identifier.urihttp://hdl.handle.net/10026.1/13202
dc.description.abstract

Biofilm-sediment aggregate (BSA) contains a high water content, either within internal pores and channels or bound by extracellular polymeric substances (EPS) forming a highly hydrated biofilm matrix. Desiccation of BSAs alters the biofilm morphology and thus the physical characteristics of porous media, such as the binding matrix within BSA and internal pore geometry. Observing BSAs in their naturally hydrated form is essential but hampered due to the lack of techniques for imaging and discerning hydrated materials. Generally, imagery techniques (scanning electron microscopy (SEM), transmission electron microscopy (TEM), and focused ion beam nanotomography (FIB-nt)) involve the desiccation of BSAs (freeze-drying or acetone dehydration) or prevent differentiation between BSA components such as inorganic particles and pore water (confocal laser scanning microscopic (CLSM)). Here, we propose a novel methodology that simultaneously achieves the 3D visualization and quantification of BSAs and their components in their hydrated form at a submicron resolution using X-ray microcomputed tomography (μ-CT). It enables the high-resolution detection of comparable morphology of multiphase components within a hydrated aggregate: each single inorganic particle and the hydrated biofilm matrix. This allows the estimation of aggregate density and the illustration of biofilm-sediment binding matrix. This information provides valuable insights into investigations of the transport of BSAs and aggregate-associated sediment particles, contaminants (such as microplastics), organic carbon, and their impacts on aquatic biogeochemical cycling.

dc.format.extent13306-13313
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subjectBiofilms
dc.subjectImaging, Three-Dimensional
dc.subjectMicroscopy, Electron, Scanning
dc.subjectPlastics
dc.subjectX-Ray Microtomography
dc.subjectX-Rays
dc.titleNondestructive 3D Imaging and Quantification of Hydrated Biofilm-Sediment Aggregates Using X-ray Microcomputed Tomography.
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/30354082
plymouth.issue22
plymouth.volume52
plymouth.publication-statusPublished
plymouth.journalEnvironmental Science and Technology
dc.identifier.doi10.1021/acs.est.8b03997
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA07 Earth Systems and Environmental Sciences
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Marine Institute
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dcterms.dateAccepted2018-10-24
dc.rights.embargodate2019-12-18
dc.identifier.eissn1520-5851
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1021/acs.est.8b03997
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-11-20
rioxxterms.typeJournal Article/Review


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