Prominin-1 controls stem cell activation by orchestrating ciliary dynamics
dc.contributor.author | Singer, D | |
dc.contributor.author | Thamm, K | |
dc.contributor.author | Zhuang, H | |
dc.contributor.author | Karbanová, J | |
dc.contributor.author | Gao, Y | |
dc.contributor.author | Walker, JV | |
dc.contributor.author | Jin, H | |
dc.contributor.author | Wu, X | |
dc.contributor.author | Coveney, CR | |
dc.contributor.author | Marangoni, P | |
dc.contributor.author | Lu, D | |
dc.contributor.author | Grayson, PRC | |
dc.contributor.author | Gulsen, T | |
dc.contributor.author | Liu, KJ | |
dc.contributor.author | Ardu, S | |
dc.contributor.author | Wann, AK | |
dc.contributor.author | Luo, Shouqing | |
dc.contributor.author | Zambon, AC | |
dc.contributor.author | Jetten, AM | |
dc.contributor.author | Tredwin, Christopher | |
dc.contributor.author | Klein, OD | |
dc.contributor.author | Attanasio, M | |
dc.contributor.author | Carmeliet, P | |
dc.contributor.author | Huttner, WB | |
dc.contributor.author | Corbeil, D | |
dc.contributor.author | Hu, Bing | |
dc.date.accessioned | 2018-12-21T21:22:10Z | |
dc.date.issued | 2018-12-06 | |
dc.identifier.issn | 0261-4189 | |
dc.identifier.issn | 1460-2075 | |
dc.identifier.other | e99845 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/13101 | |
dc.description.abstract |
Proper temporal and spatial activation of stem cells relies on highly coordinated cell signaling. The primary cilium is the sensory organelle that is responsible for transmitting extracellular signals into a cell. Primary cilium size, architecture, and assembly-disassembly dynamics are under rigid cell cycle-dependent control. Using mouse incisor tooth epithelia as a model, we show that ciliary dynamics in stem cells require the proper functions of a cholesterol-binding membrane glycoprotein, Prominin-1 (Prom1/CD133), which controls sequential recruitment of ciliary membrane components, histone deacetylase, and transcription factors. Nuclear translocation of Prom1 and these molecules is particularly evident in transit amplifying cells, the immediate derivatives of stem cells. The absence of Prom1 impairs ciliary dynamics and abolishes the growth stimulation effects of sonic hedgehog (SHH) treatment, resulting in the disruption of stem cell quiescence maintenance and activation. We propose that Prom1 is a key regulator ensuring appropriate response of stem cells to extracellular signals, with important implications for development, regeneration, and diseases. | |
dc.format.extent | 0-0 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | EMBO Press | |
dc.subject | CD133 | |
dc.subject | cilia | |
dc.subject | sonic hedgehog | |
dc.subject | stem cells | |
dc.subject | tooth | |
dc.title | Prominin-1 controls stem cell activation by orchestrating ciliary dynamics | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, N.I.H., Extramural | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/30523147 | |
plymouth.issue | 2 | |
plymouth.volume | 38 | |
plymouth.publication-status | Published | |
plymouth.journal | EMBO Journal | |
dc.identifier.doi | 10.15252/embj.201899845 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/Peninsula Dental School | |
plymouth.organisational-group | /Plymouth/Faculty of Health/Peninsula Medical School | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA03 Allied Health Professions, Dentistry, Nursing and Pharmacy | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | England | |
dcterms.dateAccepted | 2018-10-23 | |
dc.rights.embargodate | 2019-1-4 | |
dc.identifier.eissn | 1460-2075 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.15252/embj.201899845 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2018-12-06 | |
rioxxterms.type | Journal Article/Review | |
plymouth.funder | Role of the FoxN1 gene as a central regulator of epidermal planar cell polarity signaling expression and function::BBSRC | |
plymouth.funder | Role of the FoxN1 gene as a central regulator of epidermal planar cell polarity signaling expression and function::BBSRC |