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dc.contributor.authorRihan, HZen
dc.contributor.authorAl-Issawi, Men
dc.contributor.authorBurchett, Sen
dc.contributor.authorFuller, MPen
dc.date.accessioned2013-01-28T14:04:25Z
dc.date.available2013-01-28T14:04:25Z
dc.date.issued2011-11-01en
dc.identifier.issn0167-6857en
dc.identifier.urihttp://hdl.handle.net/10026.1/1268
dc.description.abstract

An effective protocol for the mass production of cauliflower microshoots was refined using the meristematic layer of cauliflower curd. After the meristematic layer was surface sterilized and shaved off, a commercial blender was used for homogenization and several blending treatments were tested in the range 15-120 s and 30 s was found to be optimal in terms of the amount explants produced and their subsequent growth ability. Explants were cultivated in S23 liquid medium (4.4 g L-1 MS (Murashige and Skoog) and 3% v/w sucrose) supplemented with several combinations of plant growth regulators (PGRs) including 1 and 2 mg L-1 of Kinetin in combination with three types of auxins (indole butyric acid (IBA), Naphthaleneacetic acid (NAA) and Indole-3-acetic acid (IAA)), each at 1 and 2 mg L-1 concentration. The use of 2 mg L-1 Kinetin and 1 mg L-1 IBA gave the best results in terms of its effects on explant induction. Microshoots of different sizes were encapsulated in a sodium alginate matrix and the optimal stage suitable for the production of artificial seeds was assessed in terms of both subsequent conversion and plantlet viability. The feasibility of cultivating cauliflower artificial seeds in commercial substrates (compost, vermiculite, perlite and sand) irrigated with different solution mixtures including sterilized distilled water (SDW), PGRs-free S23 medium and S23 medium supplemented with Kinetin (1 and 2 mg L-1) and IBA or NAA at (1 and 2 mg L-1) was investigated. The use of 2 mg L-1 Kinetin and 2 mg L-1 NAA applied with S23 gave the optimal response with both perlite and compost. This study showed high growth capacity of cauliflower artificial seeds in commercial substrates which is considered a promising step for their direct use in vivo. © 2011 Springer Science+Business Media B.V.

en
dc.format.extent243 - 250en
dc.language.isoenen
dc.titleEncapsulation of cauliflower (Brassica oleracea var botrytis) microshoots as artificial seeds and their conversion and growth in commercial substratesen
dc.typeJournal Article
plymouth.issue2en
plymouth.volume107en
plymouth.publication-statusPublisheden
plymouth.journalPlant Cell, Tissue and Organ Cultureen
dc.identifier.doi10.1007/s11240-011-9975-xen
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1007/s11240-011-9975-xen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


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