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dc.contributor.authorAl-Swedi, FGen
dc.contributor.authorLane, Sen
dc.contributor.authorFuller, MPen
dc.date.accessioned2013-01-28T14:02:25Z
dc.date.available2013-01-28T14:02:25Z
dc.date.issued2012-10en
dc.identifier.urihttp://hdl.handle.net/10026.1/1266
dc.description.abstract

Cauliflower is described as a recalcitrant plant to the genetic transformation process from Agrobacterium. Cauliflower curd meristems can be used to produce shoots to clone the mother plants in in-vitro culture. Micro propagation techniques were used in this study at different stages to produce cauliflower explants. The healthy growing explants were transformed with the APX gene using a standard transfection protocol mediated by Agrobacterium. The plants that successfully grew under abiotic stress and presenting a normal plant phenotypic feature were considered transformants. The transfection conditions were optimised during this study and 105Agrobacterium cell/5 plants/pot successfully produced 1 transformed plant/pot.

en
dc.format.extent167 - 174 (8)en
dc.languageEnglishen
dc.language.isoEnglishen
dc.publisherInternational Society of Horticultural Scienceen
dc.titleAn improved micro propagation system for successful transformation of cauliflower (Brassica oleracea var. botrytis).en
dc.typeJournal Article
plymouth.volume961en
plymouth.journalActa Horticulturaeen
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/00 Groups by role
plymouth.organisational-group/Plymouth/00 Groups by role/Academics
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
dc.rights.embargoperiodNot knownen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


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