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dc.contributor.authorAl-Swedi, FG
dc.contributor.authorLane, S
dc.contributor.authorFuller, MP
dc.date.accessioned2013-01-28T14:02:25Z
dc.date.available2013-01-28T14:02:25Z
dc.date.issued2012-10
dc.identifier.issn0567-7572
dc.identifier.issn2406-6168
dc.identifier.urihttp://hdl.handle.net/10026.1/1266
dc.description.abstract

Cauliflower is described as a recalcitrant plant to the genetic transformation process from Agrobacterium. Cauliflower curd meristems can be used to produce shoots to clone the mother plants in in-vitro culture. Micro propagation techniques were used in this study at different stages to produce cauliflower explants. The healthy growing explants were transformed with the APX gene using a standard transfection protocol mediated by Agrobacterium. The plants that successfully grew under abiotic stress and presenting a normal plant phenotypic feature were considered transformants. The transfection conditions were optimised during this study and 105Agrobacterium cell/5 plants/pot successfully produced 1 transformed plant/pot.

dc.format.extent167-174
dc.languageEnglish
dc.language.isoEnglish
dc.publisherInternational Society of Horticultural Science
dc.subjectcauliflower
dc.subjectmicro propagation
dc.subjectAgrobacterium tumefaciens
dc.titleAn improved micro propagation system for successful transformation of cauliflower (Brassica oleracea var. botrytis).
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000323607700019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue961
plymouth.volume961
plymouth.publication-statusPublished
plymouth.journalActa Horticulturae
dc.identifier.doi10.17660/actahortic.2012.961.19
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.identifier.eissn2406-6168
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.17660/actahortic.2012.961.19
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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