An improved micro propagation system for successful transformation of cauliflower (Brassica oleracea var. botrytis).
dc.contributor.author | Al-Swedi, FG | |
dc.contributor.author | Lane, S | |
dc.contributor.author | Fuller, MP | |
dc.date.accessioned | 2013-01-28T14:02:25Z | |
dc.date.available | 2013-01-28T14:02:25Z | |
dc.date.issued | 2012-10 | |
dc.identifier.issn | 0567-7572 | |
dc.identifier.issn | 2406-6168 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/1266 | |
dc.description.abstract |
Cauliflower is described as a recalcitrant plant to the genetic transformation process from Agrobacterium. Cauliflower curd meristems can be used to produce shoots to clone the mother plants in in-vitro culture. Micro propagation techniques were used in this study at different stages to produce cauliflower explants. The healthy growing explants were transformed with the APX gene using a standard transfection protocol mediated by Agrobacterium. The plants that successfully grew under abiotic stress and presenting a normal plant phenotypic feature were considered transformants. The transfection conditions were optimised during this study and 105Agrobacterium cell/5 plants/pot successfully produced 1 transformed plant/pot. | |
dc.format.extent | 167-174 | |
dc.language | English | |
dc.language.iso | English | |
dc.publisher | International Society of Horticultural Science | |
dc.subject | cauliflower | |
dc.subject | micro propagation | |
dc.subject | Agrobacterium tumefaciens | |
dc.title | An improved micro propagation system for successful transformation of cauliflower (Brassica oleracea var. botrytis). | |
dc.type | journal-article | |
dc.type | Article | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000323607700019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 961 | |
plymouth.volume | 961 | |
plymouth.publication-status | Published | |
plymouth.journal | Acta Horticulturae | |
dc.identifier.doi | 10.17660/actahortic.2012.961.19 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.identifier.eissn | 2406-6168 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.17660/actahortic.2012.961.19 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review |