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dc.contributor.authorAlghamdi, B
dc.contributor.authorfern, robert
dc.date.accessioned2018-10-16T15:01:31Z
dc.date.available2018-10-16T15:01:31Z
dc.date.issued2015-04-02
dc.identifier.issn1662-5129
dc.identifier.issn1662-5129
dc.identifier.otherARTN 49
dc.identifier.urihttp://hdl.handle.net/10026.1/12559
dc.description.abstract

The extent to which NG-2(+) cells form a distinct population separate from astrocytes is central to understanding whether this important cell class is wholly an oligodendrocyte precursor cell (OPC) or has additional functions akin to those classically ascribed to astrocytes. Early immuno-staining studies indicate that NG-2(+) cells do not express the astrocyte marker GFAP, but orthogonal reconstructions of double-labeled confocal image stacks here reveal a significant degree of co-expression in individual cells within post-natal day 10 (P10) and adult rat optic nerve (RON) and rat cortex. Extensive scanning of various antibody/fixation/embedding approaches identified a protocol for selective post-embedded immuno-gold labeling. This first ultrastructural characterization of identified NG-2(+) cells revealed populations of both OPCs and astrocytes in P10 RON. NG-2(+) astrocytes had classic features including the presence of glial filaments but low levels of glial filament expression were also found in OPCs and myelinating oligodendrocytes. P0 RONs contained few OPCs but positively identified astrocytes were observed to ensheath pre-myelinated axons in a fashion previously described as a definitive marker of the oligodendrocyte lineage. Astrocyte ensheathment was also apparent in P10 RONs, was absent from developing nodes of Ranvier and was never associated with compact myelin. Astrocyte processes were also shown to encapsulate some oligodendrocyte somata. The data indicate that common criteria for delineating astrocytes and oligodendroglia are insufficiently robust and that astrocyte features ascribed to OPCs may arise from misidentification.

dc.format.extent49-
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.language.isoeng
dc.publisherFrontiers Media SA
dc.subjectAstrocyte
dc.subjectDevelopment
dc.subjectGlia
dc.subjectOligodendrocyte
dc.subjectNG-2
dc.titlePhenotype overlap in glial cell populations: astroglia, oligodendroglia and NG-2(+) cells
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352578800002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issueMAY
plymouth.volume9
plymouth.publication-statusPublished online
plymouth.journalFrontiers in Neuroanatomy
dc.identifier.doi10.3389/fnana.2015.00049
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Admin Group - REF
plymouth.organisational-group/Plymouth/Admin Group - REF/REF Admin Group - FoH
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeSwitzerland
dcterms.dateAccepted2015-04-02
dc.identifier.eissn1662-5129
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.3389/fnana.2015.00049
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015
rioxxterms.typeJournal Article/Review
plymouth.funderIon homeostasis in optic nerve astrocytes::BBSRC


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