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dc.contributor.authorHansen, Daniel Bloch
dc.contributor.authorGarrido-Comas, N
dc.contributor.authorSalter, M
dc.contributor.authorfern, robert
dc.date.accessioned2018-10-16T15:00:49Z
dc.date.available2018-10-16T15:00:49Z
dc.date.issued2015-03
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.urihttp://hdl.handle.net/10026.1/12558
dc.description.abstract

The mechanisms of HCO3(-)-independent intracellular pH (pHi) regulation were examined in fibrous astrocytes within isolated neonatal rat optic nerve (RON) and in cultured cortical astrocytes. In agreement with previous studies, resting pHi in cultured astrocytes was 6.82 ± 0.06 and inhibition of the V-ATPase H(+) pump by Cl(-) removal or via the selective inhibitor bafilomycin had only a small effect upon resting pHi and recovery following an acid load. In contrast, resting pHi in RON astrocytes was 7.10 ± 0.04, significantly less acidic than that in cultured cells (p < 0.001), and responded to inhibition of V-ATPase with profound acidification to the 6.3-6.5 range. Fluorescent immuno-staining and immuno-gold labeling confirmed the presence V-ATPase in the cell membrane of RON astrocyte processes and somata. Using ammonia pulse recovery, pHi recovery in RON astrocyte was achieved largely via V-ATPase with sodium-proton exchange (NHE) playing a minor role. The findings indicate that astrocytes in a whole-mount preparation such as the optic nerve rely to a greater degree upon V-ATPase for HCO3(-)-independent pHi regulation than do cultured astrocytes, with important functional consequences for the regulation of pH in the CNS.

dc.format.extent8039-8047
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherElsevier BV
dc.subjectAcid Extrusion
dc.subjectAstrocyte
dc.subjectGlia
dc.subjectOptic Nerve
dc.subjectProton Transport
dc.subjectSodium-Proton Exchange
dc.subjectVacuolar ATPase
dc.subjectWhite Matter
dc.subjectpH Regulation
dc.subjectAcid-Base Equilibrium
dc.subjectAnimals
dc.subjectAstrocytes
dc.subjectBicarbonates
dc.subjectCells, Cultured
dc.subjectFemale
dc.subjectMale
dc.subjectOptic Nerve
dc.subjectRats, Wistar
dc.subjectVacuolar Proton-Translocating ATPases
dc.titleHCO3−-independent pH Regulation in Astrocytes in Situ Is Dominated by V-ATPase
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000351662600003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue13
plymouth.volume290
plymouth.publication-statusPublished
plymouth.journalJournal of Biological Chemistry
dc.identifier.doi10.1074/jbc.m115.636597
plymouth.organisational-group/Plymouth
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plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
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dc.publisher.placeUnited States
dc.identifier.eissn1083-351X
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1074/jbc.m115.636597
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
plymouth.funderIon homeostasis in optic nerve astrocytes::BBSRC


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