HCO3−-independent pH Regulation in Astrocytes in Situ Is Dominated by V-ATPase
dc.contributor.author | Hansen, Daniel Bloch | |
dc.contributor.author | Garrido-Comas, N | |
dc.contributor.author | Salter, M | |
dc.contributor.author | fern, robert | |
dc.date.accessioned | 2018-10-16T15:00:49Z | |
dc.date.available | 2018-10-16T15:00:49Z | |
dc.date.issued | 2015-03 | |
dc.identifier.issn | 0021-9258 | |
dc.identifier.issn | 1083-351X | |
dc.identifier.uri | http://hdl.handle.net/10026.1/12558 | |
dc.description.abstract |
The mechanisms of HCO3(-)-independent intracellular pH (pHi) regulation were examined in fibrous astrocytes within isolated neonatal rat optic nerve (RON) and in cultured cortical astrocytes. In agreement with previous studies, resting pHi in cultured astrocytes was 6.82 ± 0.06 and inhibition of the V-ATPase H(+) pump by Cl(-) removal or via the selective inhibitor bafilomycin had only a small effect upon resting pHi and recovery following an acid load. In contrast, resting pHi in RON astrocytes was 7.10 ± 0.04, significantly less acidic than that in cultured cells (p < 0.001), and responded to inhibition of V-ATPase with profound acidification to the 6.3-6.5 range. Fluorescent immuno-staining and immuno-gold labeling confirmed the presence V-ATPase in the cell membrane of RON astrocyte processes and somata. Using ammonia pulse recovery, pHi recovery in RON astrocyte was achieved largely via V-ATPase with sodium-proton exchange (NHE) playing a minor role. The findings indicate that astrocytes in a whole-mount preparation such as the optic nerve rely to a greater degree upon V-ATPase for HCO3(-)-independent pHi regulation than do cultured astrocytes, with important functional consequences for the regulation of pH in the CNS. | |
dc.format.extent | 8039-8047 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | eng | |
dc.publisher | Elsevier BV | |
dc.subject | Acid Extrusion | |
dc.subject | Astrocyte | |
dc.subject | Glia | |
dc.subject | Optic Nerve | |
dc.subject | Proton Transport | |
dc.subject | Sodium-Proton Exchange | |
dc.subject | Vacuolar ATPase | |
dc.subject | White Matter | |
dc.subject | pH Regulation | |
dc.subject | Acid-Base Equilibrium | |
dc.subject | Animals | |
dc.subject | Astrocytes | |
dc.subject | Bicarbonates | |
dc.subject | Cells, Cultured | |
dc.subject | Female | |
dc.subject | Male | |
dc.subject | Optic Nerve | |
dc.subject | Rats, Wistar | |
dc.subject | Vacuolar Proton-Translocating ATPases | |
dc.title | HCO3−-independent pH Regulation in Astrocytes in Situ Is Dominated by V-ATPase | |
dc.type | journal-article | |
dc.type | Journal Article | |
dc.type | Research Support, Non-U.S. Gov't | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000351662600003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 13 | |
plymouth.volume | 290 | |
plymouth.publication-status | Published | |
plymouth.journal | Journal of Biological Chemistry | |
dc.identifier.doi | 10.1074/jbc.m115.636597 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Admin Group - REF | |
plymouth.organisational-group | /Plymouth/Admin Group - REF/REF Admin Group - FoH | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/Peninsula Medical School | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | United States | |
dc.identifier.eissn | 1083-351X | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1074/jbc.m115.636597 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review | |
plymouth.funder | Ion homeostasis in optic nerve astrocytes::BBSRC |